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dc.contributor.authorDi Giovanni, Emilia
dc.contributor.authorBuonvino, Silvia
dc.contributor.authorAmelio, Ivano
dc.contributor.authorMelino, Sonia
dc.date.accessioned2020-04-04T00:46:37Z
dc.date.available2020-04-04T00:46:37Z
dc.date.issued2020-02-28
dc.identifier.issn1422-0067
dc.identifier.otherPMC7084915
dc.identifier.other32121252
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304092
dc.description.abstractThe endogenous gasotransmitter H2S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H2S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H2S-releasing agents on the growth of stem cells have not been fully investigated. H2S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H2S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H2S agents on the cell growth and differentiation of cardiac Lin- Sca1+ human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH-garlic conjugates (GSGa) on cMSC compared to other H2S-releasing agents, such as Na2S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell-cell adhesion and commitment to differentiation. These results highlight the effects of H2S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1422-0067
dc.sourcenlmid: 101092791
dc.subjectCell differentiation
dc.subjectOxidative stress
dc.subjectGarlic
dc.subjectHydrogen sulfide
dc.subjectMSCs
dc.subjectcell migration
dc.subjectRegenerative Medicine
dc.subjectOrganosulfur Compounds
dc.titleGlutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair.
dc.typeArticle
dc.date.updated2020-04-04T00:46:36Z
prism.issueIdentifier5
prism.publicationNameInternational journal of molecular sciences
prism.volume21
dc.identifier.doi10.17863/CAM.51174
rioxxterms.versionofrecord10.3390/ijms21051638
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidMelino, Sonia [0000-0001-7694-5279]


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International