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dc.contributor.authorKawaguchi, Shinichi
dc.contributor.authorUeki, Mizuki
dc.contributor.authorKai, Toshie
dc.date.accessioned2020-04-04T01:16:53Z
dc.date.available2020-04-04T01:16:53Z
dc.date.issued2020-04-03
dc.date.submitted2019-11-18
dc.identifier.otherpone-d-19-32074
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304093
dc.description.abstractMeiosis and oocyte maturation are tightly regulated processes. The meiosis arrest female 1 (MARF1) gene is essential for meiotic progression in animals; however, its detailed function remains unclear. In this study, we examined the molecular mechanism of dMarf1, a Drosophila homolog of MARF1 encoding an OST and RNA Recognition Motif (RRM) -containing protein for meiotic progression and oocyte maturation. Although oogenesis progressed in females carrying a dMarf1 loss-of-function allele, the dMarf1 mutant oocytes were found to contain arrested meiotic spindles or disrupted microtubule structures, indicating that the transition from meiosis I to II was compromised in these oocytes. The expression of the full-length dMarf1 transgene, but none of the variants lacking the OST and RRM motifs or the 47 conserved C-terminal residues among insect groups, rescued the meiotic defect in dMarf1 mutant oocytes. Our results indicate that these conserved residues are important for dMarf1 function. Immunoprecipitation of Myc-dMarf1 revealed that several mRNAs are bound to dMarf1. Of those, the protein expression of nanos (nos), but not its mRNA, was affected in the absence of dMarf1. In the control, the expression of Nos protein became downregulated during the late stages of oogenesis, while it remained high in dMarf1 mutant oocytes. We propose that dMarf1 translationally represses nos by binding to its mRNA. Furthermore, the downregulation of Nos induces cycB expression, which in turn activates the CycB/Cdk1 complex at the onset of oocyte maturation.
dc.languageen
dc.publisherPublic Library of Science
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectResearch Article
dc.subjectBiology and life sciences
dc.subjectMedicine and health sciences
dc.subjectResearch and analysis methods
dc.titleDrosophila MARF1 ensures proper oocyte maturation by regulating nanos expression
dc.typeArticle
dc.date.updated2020-04-04T01:16:52Z
prism.issueIdentifier4
prism.publicationNamePLOS ONE
prism.volume15
dc.identifier.doi10.17863/CAM.51175
dcterms.dateAccepted2020-03-16
rioxxterms.versionofrecord10.1371/journal.pone.0231114
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Mata, Juan
dc.contributor.orcidKai, Toshie [0000-0001-8675-8469]
dc.identifier.eissn1932-6203
pubs.funder-project-idJapan Society for the Promotion of Science (JP) (18H02375)
pubs.funder-project-idJapan Society for the Promotion of Science (19K06628)
pubs.funder-project-idTakeda Science Foundation (J191503009)
pubs.funder-project-idNOVARTIS Foundation (Japan) for the Promotion of Science (J191503004)
pubs.funder-project-idCollaboration with Collaborative Organizations for Female Faculties (Initiative for the Implementation of the Diversity Research Environment) (J171513002)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)