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dc.contributor.authorEsposito, Alessandro
dc.date.accessioned2020-04-06T00:29:08Z
dc.date.available2020-04-06T00:29:08Z
dc.date.issued2020-01-30
dc.identifier.issn2156-7085
dc.identifier.otherPMC7041441
dc.identifier.other32133242
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304105
dc.description.abstractFörster resonance energy transfer (FRET) imaging is an essential analytical method in biomedical research. The limited photon-budget experimentally available, however, imposes compromises between spatiotemporal and biochemical resolutions, photodamage and phototoxicity. The study of photon-statistics in biochemical imaging is thus important in guiding the efficient design of instrumentation and assays. Here, we show a comparative analysis of photon-statistics in FRET imaging demonstrating how the precision of FRET imaging varies vastly with imaging parameters. Therefore, we provide analytical and numerical tools for assay optimization. Fluorescence lifetime imaging microscopy (FLIM) is a very robust technique with excellent photon-efficiencies. However, we show that also intensity-based FRET imaging can reach high precision by utilizing information from both donor and acceptor fluorophores.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2156-7085
dc.sourcenlmid: 101540630
dc.titleHow many photons are needed for FRET imaging?
dc.typeArticle
dc.date.updated2020-04-06T00:29:08Z
prism.endingPage1202
prism.issueIdentifier2
prism.publicationNameBiomedical optics express
prism.startingPage1186
prism.volume11
dc.identifier.doi10.17863/CAM.51189
rioxxterms.versionofrecord10.1364/BOE.379305
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidEsposito, Alessandro [0000-0002-5051-091X]


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International