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dc.contributor.authorDolan, Stephen K
dc.contributor.authorKohlstedt, Michael
dc.contributor.authorTrigg, Stephen
dc.contributor.authorVallejo Ramirez, Pedro
dc.contributor.authorKaminski, Clemens F
dc.contributor.authorWittmann, Christoph
dc.contributor.authorWelch, Martin
dc.date.accessioned2020-04-19T03:14:12Z
dc.date.available2020-04-19T03:14:12Z
dc.date.issued2020-03-17
dc.identifier.issn2150-7511
dc.identifier.otherPMC7078475
dc.identifier.other32184246
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304516
dc.description.abstractPseudomonas aeruginosa is an opportunistic human pathogen, particularly noted for causing infections in the lungs of people with cystic fibrosis (CF). Previous studies have shown that the gene expression profile of P. aeruginosa appears to converge toward a common metabolic program as the organism adapts to the CF airway environment. However, we still have only a limited understanding of how these transcriptional changes impact metabolic flux at the systems level. To address this, we analyzed the transcriptome, proteome, and fluxome of P. aeruginosa grown on glycerol or acetate. These carbon sources were chosen because they are the primary breakdown products of an airway surfactant, phosphatidylcholine, which is known to be a major carbon source for P. aeruginosa in CF airways. We show that the fluxes of carbon throughout central metabolism are radically different among carbon sources. For example, the newly recognized "EDEMP cycle" (which incorporates elements of the Entner-Doudoroff [ED] pathway, the Embden-Meyerhof-Parnas [EMP] pathway, and the pentose phosphate [PP] pathway) plays an important role in supplying NADPH during growth on glycerol. In contrast, the EDEMP cycle is attenuated during growth on acetate, and instead, NADPH is primarily supplied by the reaction catalyzed by isocitrate dehydrogenase(s). Perhaps more importantly, our proteomic and transcriptomic analyses revealed a global remodeling of gene expression during growth on the different carbon sources, with unanticipated impacts on aerobic denitrification, electron transport chain architecture, and the redox economy of the cell. Collectively, these data highlight the remarkable metabolic plasticity of P. aeruginosa; that plasticity allows the organism to seamlessly segue between different carbon sources, maximizing the energetic yield from each.IMPORTANCE Pseudomonas aeruginosa is an opportunistic human pathogen that is well known for causing infections in the airways of people with cystic fibrosis. Although it is clear that P. aeruginosa is metabolically well adapted to life in the CF lung, little is currently known about how the organism metabolizes the nutrients available in the airways. In this work, we used a combination of gene expression and isotope tracer ("fluxomic") analyses to find out exactly where the input carbon goes during growth on two CF-relevant carbon sources, acetate and glycerol (derived from the breakdown of lung surfactant). We found that carbon is routed ("fluxed") through very different pathways during growth on these substrates and that this is accompanied by an unexpected remodeling of the cell's electron transfer pathways. Having access to this "blueprint" is important because the metabolism of P. aeruginosa is increasingly being recognized as a target for the development of much-needed antimicrobial agents.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2150-7511
dc.sourcenlmid: 101519231
dc.subjectPseudomonas aeruginosa
dc.subjectDenitrification
dc.subjectProteomics
dc.subjectCarbon metabolism
dc.subjectCarbon flux
dc.subjectAcetate Metabolism
dc.subjectGlycerol Metabolism
dc.titleContextual Flexibility in Pseudomonas aeruginosa Central Carbon Metabolism during Growth in Single Carbon Sources.
dc.typeArticle
dc.date.updated2020-04-19T03:14:12Z
prism.issueIdentifier2
prism.publicationNamemBio
prism.volume11
dc.identifier.doi10.17863/CAM.51596
rioxxterms.versionofrecord10.1128/mBio.02684-19
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidDolan, Stephen K [0000-0002-7391-2137]
dc.contributor.orcidVallejo Ramirez, Pedro [0000-0002-7879-6761]
dc.contributor.orcidKaminski, Clemens F [0000-0002-5194-0962]
dc.contributor.orcidWittmann, Christoph [0000-0002-7952-985X]
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/M019411/1)
pubs.funder-project-idWellcome Trust (089703/Z/09/Z)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International