A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.
Lopes Bernardes, Goncalo
Chembiochem : a European journal of chemical biology
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Kitowski, A., & Lopes Bernardes, G. (2020). A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.. Chembiochem : a European journal of chemical biology, 21 (18), 2696-2700. https://doi.org/10.1002/cbic.202000226
The introduction of chemical reporter groups in glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation. We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria. Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells. Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former. This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.
Humans, Malaria, Alkenes, Galactose, Polysaccharides, Molecular Structure, Hep G2 Cells, Metabolic Engineering
External DOI: https://doi.org/10.1002/cbic.202000226
This record's URL: https://www.repository.cam.ac.uk/handle/1810/304594
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