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dc.contributor.authorKitowski, Annabelen
dc.contributor.authorLopes Bernardes, Goncaloen
dc.date.accessioned2020-04-21T23:30:30Z
dc.date.available2020-04-21T23:30:30Z
dc.date.issued2020-09en
dc.identifier.issn1439-4227
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304594
dc.description.abstractThe introduction of chemical reporter groups in glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation. We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria. Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells. Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former. This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherWiley
dc.rightsAll rights reserved
dc.rights.uri
dc.subjectHumansen
dc.subjectMalariaen
dc.subjectAlkenesen
dc.subjectGalactoseen
dc.subjectPolysaccharidesen
dc.subjectMolecular Structureen
dc.subjectHep G2 Cellsen
dc.subjectMetabolic Engineeringen
dc.titleA Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.en
dc.typeArticle
prism.endingPage2700
prism.issueIdentifier18en
prism.publicationDate2020en
prism.publicationNameChembiochem : a European journal of chemical biologyen
prism.startingPage2696
prism.volume21en
dc.identifier.doi10.17863/CAM.51676
dcterms.dateAccepted2020-04-14en
rioxxterms.versionofrecord10.1002/cbic.202000226en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-09en
dc.contributor.orcidBernardes, Goncalo [0000-0001-6594-8917]
dc.identifier.eissn1439-7633
rioxxterms.typeJournal Article/Reviewen
cam.orpheus.successMon Apr 27 07:59:13 BST 2020 - Embargo updated*
rioxxterms.freetoread.startdate2021-04-14


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