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dc.contributor.authorGupta, Dhanak
dc.contributor.authorSilva, Melissa
dc.contributor.authorRadziun, Karolina
dc.contributor.authorMartinez, Diana C
dc.contributor.authorHill, Christopher J
dc.contributor.authorMarshall, Julie
dc.contributor.authorHearnden, Vanessa
dc.contributor.authorPuertas-Mejia, Miguel A
dc.contributor.authorReilly, Gwendolen C
dc.date.accessioned2020-04-27T23:30:18Z
dc.date.available2020-04-27T23:30:18Z
dc.date.issued2020-02-08
dc.identifier.issn1660-3397
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304717
dc.description.abstractFucoidan is a brown algae-derived polysaccharide having several biomedical applications. This study simultaneously compares the anti-cancer activities of crude fucoidans from Fucus vesiculosus and Sargassum filipendula, and effects of low (LMW, 10-50 kDa), medium (MMW, 50-100 kDa) and high (HMW, >100 kDa) molecular weight fractions of S. filipendula fucoidan against osteosarcoma cells. Glucose, fucose and acid levels were lower and sulphation was higher in F. vesiculosus crude fucoidan compared to S. filipendula crude fucoidan. MMW had the highest levels of sugars, acids and sulphation among molecular weight fractions. There was a dose-dependent drop in focal adhesion formation and proliferation of cells for all fucoidan-types, but F. vesiculosus fucoidan and HMW had the strongest effects. G1-phase arrest was induced by F. vesiculosus fucoidan, MMW and HMW, however F. vesiculosus fucoidan treatment also caused accumulation in the sub-G1-phase. Mitochondrial damage occurred for all fucoidan-types, however F. vesiculosus fucoidan led to mitochondrial fragmentation. Annexin V/PI, TUNEL and cytochrome c staining confirmed stress-induced apoptosis-like cell death for F. vesiculosus fucoidan and features of stress-induced necrosis-like cell death for S. filipendula fucoidans. There was also variation in penetrability of different fucoidans inside the cell. These differences in anti-cancer activity of fucoidans are applicable for osteosarcoma treatment.
dc.format.mediumElectronic
dc.languageeng
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCell Line, Tumor
dc.subjectMitochondria
dc.subjectHumans
dc.subjectFucus
dc.subjectSargassum
dc.subjectOsteosarcoma
dc.subjectPolysaccharides
dc.subjectCell Cycle
dc.subjectApoptosis
dc.subjectMolecular Weight
dc.subjectPhaeophyta
dc.titleFucoidan Inhibition of Osteosarcoma Cells Is Species and Molecular Weight Dependent.
dc.typeArticle
prism.issueIdentifier2
prism.publicationDate2020
prism.publicationNameMar Drugs
prism.volume18
dc.identifier.doi10.17863/CAM.51798
dcterms.dateAccepted2020-02-05
rioxxterms.versionofrecord10.3390/md18020104
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-02-08
dc.contributor.orcidGupta, Dhanak [0000-0002-6493-1600]
dc.contributor.orcidHill, Christopher J [0000-0002-6914-4411]
dc.contributor.orcidHearnden, Vanessa [0000-0003-0838-7783]
dc.contributor.orcidPuertas-Mejia, Miguel A [0000-0002-9962-669X]
dc.contributor.orcidReilly, Gwendolen C [0000-0003-1456-1071]
dc.identifier.eissn1660-3397
rioxxterms.typeJournal Article/Review
cam.issuedOnline2020-02-08


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International