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dc.contributor.authorSingh, Vikash
dc.contributor.authorDavidson, Anthony C
dc.contributor.authorHume, Peter J
dc.contributor.authorKoronakis, Vassilis
dc.date.accessioned2020-05-09T00:30:23Z
dc.date.available2020-05-09T00:30:23Z
dc.date.issued2020-04-02
dc.identifier.issn1422-0067
dc.identifier.otherPMC7177560
dc.identifier.other32252226
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/305158
dc.description.abstractThe small GTPase ADP-ribosylation factor 6 (Arf6) anchors at the plasma membrane to orchestrate key functions, such as membrane trafficking and regulating cortical actin cytoskeleton rearrangement. A number of studies have identified key players that interact with Arf6 to regulate actin dynamics in diverse cell processes, yet it is still unknown whether Arf6 can directly signal to the wave regulatory complex to mediate actin assembly. By reconstituting actin dynamics on supported lipid bilayers, we found that Arf6 in co-ordination with Rac1(Ras-related C3 botulinum toxin substrate 1) can directly trigger actin polymerization by recruiting wave regulatory complex components. Interestingly, we demonstrated that Arf6 triggers actin assembly at the membrane directly without recruiting the Arf guanine nucleotide exchange factor (GEF) ARNO (ARF nucleotide-binding site opener), which is able to activate Arf1 to enable WRC-dependent actin assembly. Furthermore, using labelled E. coli, we demonstrated that actin assembly by Arf6 also contributes towards efficient phagocytosis in THP-1 macrophages. Taken together, this study reveals a mechanism for Arf6-driven actin polymerization.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1422-0067
dc.sourcenlmid: 101092791
dc.subjectMacrophages
dc.subjectPhagocytosis
dc.subjectActin cytoskeleton
dc.subjectArf Gtpases
dc.subjectWave Regulatory Complex
dc.subjectHost–pathogen Interplay
dc.titleArf6 Can Trigger Wave Regulatory Complex-Dependent Actin Assembly Independent of Arno.
dc.typeArticle
dc.date.updated2020-05-09T00:30:23Z
prism.issueIdentifier7
prism.publicationNameInternational journal of molecular sciences
prism.volume21
dc.identifier.doi10.17863/CAM.52239
rioxxterms.versionofrecord10.3390/ijms21072457
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
pubs.funder-project-idMedical Research Council (MR/L008122/1)
pubs.funder-project-idWellcome Trust (101828/Z/13/Z)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International