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dc.contributor.authorKanvatirth, Panchali
dc.contributor.authorRossi, O
dc.contributor.authorRestif, Olivier
dc.contributor.authorBlacklaws, Barbara
dc.contributor.authorTonks, Paul
dc.contributor.authorGrant, Andrew
dc.contributor.authorMastroeni, Pietro
dc.date.accessioned2020-05-12T23:30:19Z
dc.date.available2020-05-12T23:30:19Z
dc.date.issued2020-10-01
dc.identifier.issn0305-7453
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/305310
dc.description.abstractOBJECTIVES: To determine the immune cell populations associated with Salmonella enterica serovar Typhimurium before and after ciprofloxacin treatment using a murine model of systemic infection. The effect of depletion of immune cells associating with Salmonella on treatment outcome was also determined. METHODS: We infected mice with a Salmonella enterica serovar Typhimurium strain expressing GFP and used multicolour flow cytometry to identify splenic immune cell populations associating with GFP-positive Salmonella before and after treatment with ciprofloxacin. This was followed by depletion of different immune cell populations using antibodies and liposomes. RESULTS: Our results identified CD11b+CD11chi/lo (dendritic cells/macrophages) and Ly6G+CD11b+ (neutrophils) leucocytes as the main host cell populations that are associated with Salmonella after ciprofloxacin treatment. We therefore proceeded to test the effects of depletion of such populations during treatment. We show that depletion of Ly6G+CD11b+ populations resulted in an increase in the number of viable bacterial cells in the spleen at the end of ciprofloxacin treatment. Conversely, treatment with clodronate liposomes during antimicrobial treatment, which depleted the CD11b+CD11chi/lo populations, resulted in lower numbers of viable bacteria in the tissues. CONCLUSIONS: Our study identified host cells where Salmonella bacteria persist during ciprofloxacin treatment and revealed a dual and opposing effect of removal of Ly6G+CD11b+ and CD11b+CD11chi/lo host cells on the efficacy of antimicrobial treatment. This suggests a dichotomy in the role of these populations in clearance/persistence of Salmonella during antimicrobial treatment.
dc.format.mediumPrint
dc.languageeng
dc.publisherOxford University Press (OUP)
dc.rightsAll rights reserved
dc.subjectSpleen
dc.subjectNeutrophils
dc.subjectAnimals
dc.subjectMice
dc.subjectSalmonella enterica
dc.subjectSalmonella Infections
dc.subjectSalmonella Infections, Animal
dc.subjectCiprofloxacin
dc.titleDual role of splenic mononuclear and polymorphonuclear cells in the outcome of ciprofloxacin treatment of Salmonella enterica infections.
dc.typeArticle
prism.endingPage2918
prism.issueIdentifier10
prism.publicationDate2020
prism.publicationNameJ Antimicrob Chemother
prism.startingPage2914
prism.volume75
dc.identifier.doi10.17863/CAM.52395
dcterms.dateAccepted2020-05-11
rioxxterms.versionofrecord10.1093/jac/dkaa249
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-10
dc.contributor.orcidKanvatirth, Panchali [0000-0002-1189-7832]
dc.contributor.orcidRestif, Olivier [0000-0001-9158-853X]
dc.contributor.orcidBlacklaws, Barbara [0000-0001-5454-6496]
dc.contributor.orcidTonks, Paul [0000-0003-3512-5631]
dc.contributor.orcidGrant, Andrew [0000-0001-9746-2989]
dc.contributor.orcidMastroeni, Pietro [0000-0003-3838-4962]
dc.identifier.eissn1460-2091
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/M000982/1)
cam.issuedOnline2020-07-02
cam.orpheus.successMon Jul 20 07:54:47 BST 2020 - Embargo updated
cam.orpheus.counter11
rioxxterms.freetoread.startdate2021-07-31


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