Intraarticular Adeno-Associated Virus Serotype AAV-PHP.S-Mediated Chemogenetic Targeting of Knee-Innervating Dorsal Root Ganglion Neurons Alleviates Inflammatory Pain in Mice.
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Authors
Chakrabarti, Sampurna
Pattison, Luke
Doleschall, Balint
Rickman, Rebecca H
Blake, Helen
Callejo, Gerard
Heppenstall, Paul A
Publication Date
2020-10Journal Title
Arthritis Rheumatol
ISSN
2326-5191
Publisher
Wiley
Volume
72
Issue
10
Pages
1749-1758
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Chakrabarti, S., Pattison, L., Doleschall, B., Rickman, R. H., Blake, H., Callejo, G., Heppenstall, P. A., & et al. (2020). Intraarticular Adeno-Associated Virus Serotype AAV-PHP.S-Mediated Chemogenetic Targeting of Knee-Innervating Dorsal Root Ganglion Neurons Alleviates Inflammatory Pain in Mice.. Arthritis Rheumatol, 72 (10), 1749-1758. https://doi.org/10.1002/art.41314
Abstract
OBJECTIVE: Joint pain is the major clinical symptom of arthritis that affects millions of people. Controlling the excitability of knee-innervating dorsal root ganglion (DRG) neurons (knee neurons) could potentially provide pain relief. We undertook this study to evaluate whether the newly engineered adeno-associated virus (AAV) serotype, AAV-PHP.S, can deliver functional artificial receptors to control knee neuron excitability following intraarticular knee injection. METHODS: The AAV-PHP.S virus, packaged with dTomato fluorescent protein and either excitatory (Gq ) or inhibitory (Gi ) designer receptors exclusively activated by designer drugs (DREADDs), was injected into the knee joints of adult mice. Labeling of DRG neurons with AAV-PHP.S from the knee was evaluated using immunohistochemistry. The functionality of Gq - and Gi -DREADDs was evaluated using whole-cell patch clamp electrophysiology on acutely cultured DRG neurons. Pain behavior in mice was assessed using a digging assay, dynamic weight bearing, and rotarod performance, before and after intraperitoneal administration of the DREADD activator, Compound 21. RESULTS: We showed that AAV-PHP.S can deliver functional genes into ~7% of lumbar DRG neurons when injected into the knee joint in a similar manner to the well-established retrograde tracer, fast blue. Short-term activation of AAV-PHP.S-delivered Gq -DREADD increased excitability of knee neurons in vitro (P = 0.02 by unpaired t-test), without inducing overt pain in mice when activated in vivo. By contrast, in vivo Gi -DREADD activation alleviated digging deficits induced by Freund's complete adjuvant-mediated knee inflammation (P = 0.0002 by repeated-measures analysis of variance [ANOVA] followed by Holm-Sidak multiple comparisons test). A concomitant decrease in knee neuron excitability was observed in vitro (P = 0.005 by ANOVA followed by Holm-Sidak multiple comparisons test). CONCLUSION: We describe an AAV-mediated chemogenetic approach to specifically control joint pain, which may be utilized in translational arthritic pain research.
Keywords
Knee Joint, Ganglia, Spinal, Neurons, Animals, Mice, Dependovirus, Pain, Disease Models, Animal, Inflammation, Pain Management, Genetic Therapy
Relationships
Is supplemented by: https://doi.org/10.17863/CAM.46171
Sponsorship
This study was supported by Versus Arthritis Project grants (RG 20930 and RG 21973) to E. St. J. S. and G.C. S.C. was supported by Gates Cambridge Trust scholarship, Cambridge Philosophical Society research fund, Department of Pharmacology (University of Cambridge) travel award and Corpus Christi College (University of Cambridge) travel award. L.A.P. was supported by the University of Cambridge BBSRC Doctoral Training Programme (BB/M011194/1). P.A.H and B.D. were supported by EMBL.
Funder references
ARTHRITIS RESEARCH UK (20930)
Arthritis Research UK (11600/21973)
Biotechnology and Biological Sciences Research Council (BB/M011194/1)
Identifiers
External DOI: https://doi.org/10.1002/art.41314
This record's URL: https://www.repository.cam.ac.uk/handle/1810/305329
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