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dc.contributor.authorMookerjee, S
dc.contributor.authorFoster, HR
dc.contributor.authorWaller, AK
dc.contributor.authorGhevaert, CJ
dc.date.accessioned2020-05-13T23:30:52Z
dc.date.available2020-05-13T23:30:52Z
dc.date.issued2020-08-17
dc.identifier.issn0953-7104
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/305377
dc.description.abstractPlatelet transfusions are given to patients in hospital who have a low blood platelet count (thrombocytopenia) either because of major bleeding (following trauma or surgery) or because the bone marrow production of platelets is impaired often due to chemotherapy, infiltration with malignant cells, fibrosis or genetic disorders. We are currently entirely reliant on blood donors as a source of platelets in transfusion medicine. However, the demand for platelets continues to rise, driven by an aging population, advances in medical procedures and ever more aggressive cancer therapies, while the supply of blood donors continues to remain static. In recent years, several groups have made major advances toward the generation of platelets in vitro for human transfusion. Recent successes include results in both generating mature human megakaryocytes as well as in developing bioreactors for extracting platelets from these megakaryocytes. Platelets made in vitro could address several issues inherent to platelets derived from blood donors - the ability to scale up/down more flexibly according to demand and therefore less precarious supply line, reduction of the risk of exposure to infectious agents and finally the possibility of engineering stem cells to reduce immunogenicity. Here we define the quality control tools and suggest measures for implementation across the field for in vitro platelet genesis, to aid collaboration between laboratories and to aid production of the burdens of proof that will eventually be required by regulators for efficacy and biosafety. We will do this firstly, by addressing the quality control of the nucleated cells used to make the platelets with a particular emphasis to safety issues and secondly, we will look at how platelet function measurement are addressed particularly in the context of platelets derived in vitro.
dc.description.sponsorshipThis work was supported by grants from the Rosetrees Trust (A1691), NHS Blood and Transplant and the European Union (SilkFusion: AMD-767309-3).
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherInforma UK Limited
dc.rightsAll rights reserved
dc.subjectBlood Platelets
dc.subjectHumans
dc.subjectCell Culture Techniques
dc.titleIn vitro-derived platelets: the challenges we will have to face to assess quality and safety.
dc.typeArticle
prism.endingPage730
prism.issueIdentifier6
prism.publicationDate2020
prism.publicationNamePlatelets
prism.startingPage724
prism.volume31
dc.identifier.doi10.17863/CAM.52460
dcterms.dateAccepted2020-05-09
rioxxterms.versionofrecord10.1080/09537104.2020.1769051
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-08
dc.contributor.orcidMookerjee, S [0000-0003-4904-1324]
dc.contributor.orcidFoster, HR [0000-0001-8118-9842]
dc.contributor.orcidWaller, AK [0000-0002-9726-5560]
dc.contributor.orcidGhevaert, CJ [0000-0002-9251-0934]
dc.identifier.eissn1369-1635
rioxxterms.typeJournal Article/Review
pubs.funder-project-idRosetrees Trust (A1691)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) Future and Emerging Technologies (FET) (767309)
pubs.funder-project-idMedical Research Council (MC_PC_12009)
pubs.funder-project-idMedical Research Council (MC_PC_17230)
cam.issuedOnline2020-06-02
cam.orpheus.successMon Jun 08 08:21:36 BST 2020 - Embargo updated
cam.orpheus.counter3
rioxxterms.freetoread.startdate2021-06-02


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