This data is made of z-stacks obtain by confocal microscopy. In this data set, you will find six folders. Each folder is named after the figure number in the original publication. One folder corresponds to one figure of the paper with the microscopy data for that figure; for example, the folder named figure2 contains all the microscopy data for figure2 of the article. Within each figure folder, you will find subfolders classified by the category of the data. Each figure folder contains subfolders. The first subfolder is named after the type of cytoskeletal element that was imaged (actin or microtubules). The next subfolder corresponds to which shape the cells were confined in (controls, rectangles, squares, triangles or circles). The last subfolder is the date the images were taken and contains all the files obtained that day. Each file corresponds to one cell and is named stack_c1.czi, stack_c2.czi ... for every cell imaged that day. Every file is a collection of focus plan acquired with the z-stack mode of the Zen software (Zeiss) and these files can be opened with Fiji software. Further details about the image analysis and the data analysis for the associated article can be found on the GitLab page of the publication: https://gitlab.com/slcu/teamHJ/publications/durand_etal_2019/-/tree/master.