A direct role for SNX9 in the biogenesis of filopodia.
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Authors
Jarsch, Iris K
Nuccitelli, Annalisa
Mason, Julia
Shimo, Hanae
Pilloux, Ludovic
Marzook, Bishara
Mulvey, Claire M
Dobramysl, Ulrich
Bradshaw, Charles R
Lilley, Kathryn S
Hayward, Richard D
Vaughan, Tristan J
Dobson, Claire L
Gallop, Jennifer L
Publication Date
2020-04-01Journal Title
The Journal of cell biology
ISSN
0021-9525
Volume
219
Issue
4
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Jarsch, I. K., Gadsby, J. R., Nuccitelli, A., Mason, J., Shimo, H., Pilloux, L., Marzook, B., et al. (2020). A direct role for SNX9 in the biogenesis of filopodia.. The Journal of cell biology, 219 (4)https://doi.org/10.1083/jcb.201909178
Abstract
Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell-cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia.
Sponsorship
Wellcome Trust (105602/Z/14/Z, 108467/Z/15/Z, 092096)
European Research Council (281971)
Identifiers
PMC7147113, 32328641
External DOI: https://doi.org/10.1083/jcb.201909178
This record's URL: https://www.repository.cam.ac.uk/handle/1810/305736