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dc.contributor.authorRoth, Ronelle
dc.contributor.authorHillmer, Stefan
dc.contributor.authorFunaya, Charlotta
dc.contributor.authorChiapello, Marco
dc.contributor.authorSchumacher, Karin
dc.contributor.authorLo Presti, Libera
dc.contributor.authorKahmann, Regine
dc.contributor.authorPaszkowski, Uta
dc.date.accessioned2020-05-29T10:57:07Z
dc.date.available2020-05-29T10:57:07Z
dc.date.issued2019-02
dc.identifier.issn2055-0278
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/305876
dc.description.abstractDuring establishment of arbuscular mycorrhizal symbioses, fungal hyphae invade root cells producing transient tree-like structures, the arbuscules, where exchange of photosynthates for soil minerals occurs. Arbuscule formation and collapse lead to rapid production and degradation of plant and fungal membranes, their spatiotemporal dynamics directly influencing nutrient exchange. We determined the ultra-structural details of both membrane surfaces and the interstitial apoplastic matrix by transmission electron microscopy tomography during growth and senescence of Rhizophagus irregularis arbuscules in rice. Invasive growth of arbuscular hyphae was associated with abundant fungal membrane tubules (memtubs) and plant peri-arbuscular membrane evaginations. Similarly, the phylogenetically distant arbuscular mycorrhizal fungus, Gigaspora rosea, and the fungal maize pathogen, Ustilago maydis, developed memtubs while invading host cells, revealing structural commonalities independent of the mutualistic or parasitic outcome of the interaction. Additionally, extracellular vesicles formed continuously in the peri-arbuscular interface from arbuscule biogenesis to senescence, suggesting an involvement in inter-organismic signal and nutrient exchange throughout the arbuscule lifespan.
dc.description.sponsorshipR. Roth was supported by Marie Curie FP7-PEOPLE-2013-IEF grant No. 629887 and by the Isaac Newton Trust RG74108; and U. Paszkowski by the BBSRC grant No. BB/N008723/1.
dc.languageeng
dc.publisherSpringer Nature
dc.rightsAll rights reserved
dc.subjectCell Membrane
dc.subjectElectron Microscope Tomography
dc.subjectExtracellular Vesicles
dc.subjectGlomeromycota
dc.subjectHyphae
dc.subjectMycorrhizae
dc.subjectOryza
dc.subjectPlant Cells
dc.subjectPlant Leaves
dc.subjectPlant Roots
dc.subjectPlants, Genetically Modified
dc.subjectSymbiosis
dc.subjectUstilago
dc.subjectZea mays
dc.titleArbuscular cell invasion coincides with extracellular vesicles and membrane tubules.
dc.typeArticle
prism.endingPage211
prism.issueIdentifier2
prism.publicationDate2019
prism.publicationNameNature Plants
prism.startingPage204
prism.volume5
dc.identifier.doi10.17863/CAM.52956
dcterms.dateAccepted2019-01-08
rioxxterms.versionofrecord10.1038/s41477-019-0365-4
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-02
dc.contributor.orcidPaszkowski, Uta [0000-0002-7279-7632]
dc.identifier.eissn2055-0278
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/N008723/1)
pubs.funder-project-idEuropean Commission (629887)
cam.issuedOnline2019-02-08


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