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dc.contributor.authorRoth, Ronelleen
dc.contributor.authorHillmer, Stefanen
dc.contributor.authorFunaya, Charlottaen
dc.contributor.authorChiapello, Marcoen
dc.contributor.authorSchumacher, Karinen
dc.contributor.authorLo Presti, Liberaen
dc.contributor.authorKahmann, Regineen
dc.contributor.authorPaszkowski, Utaen
dc.date.accessioned2020-05-29T10:57:07Z
dc.date.available2020-05-29T10:57:07Z
dc.date.issued2019-02en
dc.identifier.issn2055-0278
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/305876
dc.description.abstractDuring establishment of arbuscular mycorrhizal symbioses, fungal hyphae invade root cells producing transient tree-like structures, the arbuscules, where exchange of photosynthates for soil minerals occurs. Arbuscule formation and collapse lead to rapid production and degradation of plant and fungal membranes, their spatiotemporal dynamics directly influencing nutrient exchange. We determined the ultra-structural details of both membrane surfaces and the interstitial apoplastic matrix by transmission electron microscopy tomography during growth and senescence of Rhizophagus irregularis arbuscules in rice. Invasive growth of arbuscular hyphae was associated with abundant fungal membrane tubules (memtubs) and plant peri-arbuscular membrane evaginations. Similarly, the phylogenetically distant arbuscular mycorrhizal fungus, Gigaspora rosea, and the fungal maize pathogen, Ustilago maydis, developed memtubs while invading host cells, revealing structural commonalities independent of the mutualistic or parasitic outcome of the interaction. Additionally, extracellular vesicles formed continuously in the peri-arbuscular interface from arbuscule biogenesis to senescence, suggesting an involvement in inter-organismic signal and nutrient exchange throughout the arbuscule lifespan.
dc.description.sponsorshipR. Roth was supported by Marie Curie FP7-PEOPLE-2013-IEF grant No. 629887 and by the Isaac Newton Trust RG74108; and U. Paszkowski by the BBSRC grant No. BB/N008723/1.
dc.languageengen
dc.publisherSpringer Nature
dc.rightsAll rights reserved
dc.subjectCell Membraneen
dc.subjectElectron Microscope Tomographyen
dc.subjectExtracellular Vesiclesen
dc.subjectGlomeromycotaen
dc.subjectHyphaeen
dc.subjectMycorrhizaeen
dc.subjectOryzaen
dc.subjectPlant Cellsen
dc.subjectPlant Leavesen
dc.subjectPlant Rootsen
dc.subjectPlants, Genetically Modifieden
dc.subjectSymbiosisen
dc.subjectUstilagoen
dc.subjectZea maysen
dc.titleArbuscular cell invasion coincides with extracellular vesicles and membrane tubules.en
dc.typeArticle
prism.endingPage211
prism.issueIdentifier2en
prism.publicationDate2019en
prism.publicationNameNature Plantsen
prism.startingPage204
prism.volume5en
dc.identifier.doi10.17863/CAM.52956
dcterms.dateAccepted2019-01-08en
rioxxterms.versionofrecord10.1038/s41477-019-0365-4en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-02en
dc.contributor.orcidPaszkowski, Uta [0000-0002-7279-7632]
dc.identifier.eissn2055-0278
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/N008723/1)
pubs.funder-project-idEuropean Commission (629887)
cam.issuedOnline2019-02-08en


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