Toxicity and biofilm-based selection for methyl tert-butyl ether bioremediation technology.
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Publication Date
2016-12Journal Title
Water Sci Technol
ISSN
0273-1223
Publisher
IWA Publishing
Volume
74
Issue
12
Pages
2889-2897
Language
eng
Type
Article
This Version
AM
Physical Medium
Print
Metadata
Show full item recordCitation
Guisado, I., Purswani, J., Catón-Alcubierre, L., González-López, J., & Pozo, C. (2016). Toxicity and biofilm-based selection for methyl tert-butyl ether bioremediation technology.. Water Sci Technol, 74 (12), 2889-2897. https://doi.org/10.2166/wst.2016.461
Abstract
Extractive membrane biofilm reactor (EMBFR) technology offers productive solutions for volatile and semi-volatile compound removal from water bodies. In this study, the bacterial strains Paenibacillus etheri SH7T (CECT 8558), Agrobacterium sp. MS2 (CECT 8557) and Rhodococcus ruber strains A5 (CECT 8556), EE6 (CECT 8612) and EE1 (CECT 8555), previously isolated from fuel-contaminated sites, were tested for adherence on tubular semipermeable membranes in laboratory-scale systems designed for methyl tert-butyl ether (MTBE) bioremediation. Biofilm formation on the membrane surface was evaluated through observation by field-emission scanning electron microscope (FESEM) as well as the acute toxicity (as EC50) of the bacterial growth media. Moreover, extracellular polymeric substance (EPS) production for each strain under different MTBE concentrations was measured. Strains A5 and MS2 were biofilm producers and their adherence increased when the MTBE flowed through the inner tubular semipermeable membrane. No biofilm was formed by Paenibacillus etheri SH7T, nevertheless, the latter and strain MS2 exhibited the lowest toxicity after growth on the EMBFR. The results obtained from FESEM and toxicity analysis demonstrate that bacterial strains R. ruber EE6, A5, P. etheri SH7T and Agrobacterium sp. MS2 could be excellent candidates to be used as selective inocula in EMBFR technology for MTBE bioremediation.
Keywords
Biofilms, Rhodococcus, Methyl Ethers, Culture Media, Biodegradation, Environmental, Paenibacillus, Agrobacterium
Identifiers
External DOI: https://doi.org/10.2166/wst.2016.461
This record's URL: https://www.repository.cam.ac.uk/handle/1810/306038
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