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Proteomic analysis of meiosis and characterization of novel short open reading frames in the fission yeast Schizosaccharomyces pombe.

Accepted version
Peer-reviewed

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Authors

Huraiova, Barbora 
Kanovits, Judit 
Polakova, Silvia Bagelova  ORCID logo  https://orcid.org/0000-0002-7461-9071

Abstract

Meiosis is the process by which haploid gametes are produced from diploid precursor cells. We used stable isotope labeling by amino acids in cell culture (SILAC) to characterize the meiotic proteome in the fission yeast Schizosaccharomyces pombe. We compared relative levels of proteins extracted from cells harvested around meiosis I with those of meiosis II, and proteins from premeiotic S phase with the interval between meiotic divisions, when S phase is absent. Our proteome datasets revealed peptides corresponding to short open reading frames (sORFs) that have been previously identified by ribosome profiling as new translated regions. We verified expression of selected sORFs by Western blotting and analyzed the phenotype of deletion mutants. Our data provide a resource for studying meiosis that may help understand differences between meiosis I and meiosis II and how S phase is suppressed between the two meiotic divisions.

Description

Keywords

Meiosis, fission yeast Schizosaccharomyces pombe, short open reading frames, silac, Gene Deletion, Isotope Labeling, Meiosis, Open Reading Frames, Phenotype, Proteome, Proteomics, Reproducibility of Results, Ribosomes, Schizosaccharomyces, Schizosaccharomyces pombe Proteins

Journal Title

Cell Cycle

Conference Name

Journal ISSN

1538-4101
1551-4005

Volume Title

19

Publisher

Informa UK Limited

Rights

All rights reserved
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/M021483/1)