Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Minshall, Nicola https://orcid.org/0000-0002-8350-4903
Git, Anna https://orcid.org/0000-0003-0973-9138
Abstract
Reverse transcription is the first step of most analyses of gene expression, yet the quantitative biases it introduces are largely overlooked. Following a series of purpose-designed systematic experiments we cherry-pick examples of various biases introduced by reverse transcription, and alert the "gene expression community" to the pitfalls and improved practice of this fundamental technique.
Description
Keywords
Cell Line, Tumor, Gene Expression Profiling, Humans, RNA, Real-Time Polymerase Chain Reaction, Reverse Transcription
Journal Title
Sci Rep
Conference Name
Journal ISSN
2045-2322
2045-2322
2045-2322
Volume Title
10
Publisher
Springer Science and Business Media LLC
Publisher DOI
Sponsorship
Breast Cancer Now (2016NovPR816)