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dc.contributor.authorBayraktar, Omer Ali
dc.contributor.authorBartels, Theresa
dc.contributor.authorHolmqvist, Staffan
dc.contributor.authorKleshchevnikov, Vitalii
dc.contributor.authorMartirosyan, Araks
dc.contributor.authorPolioudakis, Damon
dc.contributor.authorBen Haim, Lucile
dc.contributor.authorYoung, Adam MH
dc.contributor.authorBatiuk, Mykhailo Y
dc.contributor.authorPrakash, Kirti
dc.contributor.authorBrown, Alexander
dc.contributor.authorRoberts, Kenny
dc.contributor.authorParedes, Mercedes F
dc.contributor.authorKawaguchi, Riki
dc.contributor.authorStockley, John
dc.contributor.authorSabeur, Khalida
dc.contributor.authorChang, Sandra M
dc.contributor.authorHuang, Eric
dc.contributor.authorHutchinson, Peter
dc.contributor.authorUllian, Erik M
dc.contributor.authorHemberg, Martin
dc.contributor.authorCoppola, Giovanni
dc.contributor.authorHolt, Matthew G
dc.contributor.authorGeschwind, Daniel H
dc.contributor.authorRowitch, David
dc.date.accessioned2020-06-18T13:24:41Z
dc.date.available2020-06-18T13:24:41Z
dc.date.issued2020-04
dc.identifier.issn1097-6256
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/306919
dc.description.abstractAlthough the cerebral cortex is organized into six excitatory neuronal layers, it is unclear whether glial cells show distinct layering. In the present study, we developed a high-content pipeline, the large-area spatial transcriptomic (LaST) map, which can quantify single-cell gene expression in situ. Screening 46 candidate genes for astrocyte diversity across the mouse cortex, we identified superficial, mid and deep astrocyte identities in gradient layer patterns that were distinct from those of neurons. Astrocyte layer features, established in the early postnatal cortex, mostly persisted in adult mouse and human cortex. Single-cell RNA sequencing and spatial reconstruction analysis further confirmed the presence of astrocyte layers in the adult cortex. Satb2 and Reeler mutations that shifted neuronal post-mitotic development were sufficient to alter glial layering, indicating an instructive role for neuronal cues. Finally, astrocyte layer patterns diverged between mouse cortical regions. These findings indicate that excitatory neurons and astrocytes are organized into distinct lineage-associated laminae.
dc.description.sponsorshipThe study was supported by the Paul G. Allen Foundation Distinguished Investigator Program (E.M.U. and D.H.R.), the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation (D.H.R., D.G. and G. C.), BRAIN initiative (1U01 MH105991 to D.G.) and National Institute of Health (1R01 MH109912 to D.G.; P01NS08351 to D.H.R.), National Institute of Health Research and the European Union Seventh Framework (to P.H.), NINDS Informatics Center for Neurogenetics and Neurogenomics (P30 NS062691 to G.C.), Wellcome Trust core support (M.H., O.A.B.), European Research Council (281961 to M.G.H.), Fonds Wetenschappelijk Onderzoek (G066715N and 1523014N to M.G.H.), Stichting Alzheimer Onderzoek (S#16025 to M.G.H.) and VIB Institutional Support and Tech Watch funding (to M.G.H.), Howard Hughes Medical Institute and the Wellcome Trust (to D.H.R.).
dc.format.mediumPrint-Electronic
dc.languageeng
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.rightsAll rights reserved
dc.subjectCerebral Cortex
dc.subjectAstrocytes
dc.subjectNeurons
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectBrain Mapping
dc.subjectTranscriptome
dc.titleAstrocyte layers in the mammalian cerebral cortex revealed by a single-cell in situ transcriptomic map.
dc.typeArticle
prism.endingPage509
prism.issueIdentifier4
prism.publicationDate2020
prism.publicationNameNat Neurosci
prism.startingPage500
prism.volume23
dc.identifier.doi10.17863/CAM.54012
dcterms.dateAccepted2020-01-28
rioxxterms.versionofrecord10.1038/s41593-020-0602-1
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-04
dc.contributor.orcidBayraktar, Omer Ali [0000-0001-6055-277X]
dc.contributor.orcidBartels, Theresa [0000-0003-4100-0608]
dc.contributor.orcidHolmqvist, Staffan [0000-0001-6709-6666]
dc.contributor.orcidKleshchevnikov, Vitalii [0000-0001-9110-7441]
dc.contributor.orcidBatiuk, Mykhailo Y [0000-0001-6681-4048]
dc.contributor.orcidPrakash, Kirti [0000-0002-0325-9988]
dc.contributor.orcidRoberts, Kenny [0000-0001-6155-0821]
dc.contributor.orcidStockley, John [0000-0002-7385-8310]
dc.contributor.orcidHuang, Eric [0000-0002-5381-3801]
dc.contributor.orcidHutchinson, Peter [0000-0002-2796-1835]
dc.contributor.orcidHemberg, Martin [0000-0001-8895-5239]
dc.contributor.orcidHolt, Matthew G [0000-0002-8958-4027]
dc.contributor.orcidGeschwind, Daniel H [0000-0003-2896-3450]
dc.contributor.orcidRowitch, David [0000-0002-0079-0060]
dc.identifier.eissn1546-1726
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (108139/B/15/Z)
pubs.funder-project-idDr. Miriam and Sheldon G. Adelson Medical Research Foundation (AMRF) (EQ2017B)
pubs.funder-project-idWellcome Trust (108139/Z/15/Z)
pubs.funder-project-idMedical Research Council (MC_PC_12009)
pubs.funder-project-idMedical Research Council (MC_PC_17230)
pubs.funder-project-idEuropean Research Council (789054)
pubs.funder-project-idWellcome Trust (109142/Z/15/Z)
cam.issuedOnline2020-03-16
cam.orpheus.successMon Jun 22 08:16:53 BST 2020 - Embargo updated
rioxxterms.freetoread.startdate2020-10-30


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