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dc.contributor.authorKontopoulos, Ioannis
dc.contributor.authorPenkman, Kirsty
dc.contributor.authorMullin, Victoria E.
dc.contributor.authorWinkelbach, Laura
dc.contributor.authorUnterländer, Martina
dc.contributor.authorScheu, Amelie
dc.contributor.authorKreutzer, Susanne
dc.contributor.authorHansen, Henrik B.
dc.contributor.authorMargaryan, Ashot
dc.contributor.authorTeasdale, Matthew D.
dc.contributor.authorGehlen, Birgit
dc.contributor.authorStreet, Martin
dc.contributor.authorLynnerup, Niels
dc.contributor.authorLiritzis, Ioannis
dc.contributor.authorSampson, Adamantios
dc.contributor.authorPapageorgopoulou, Christina
dc.contributor.authorAllentoft, Morten E.
dc.contributor.authorBurger, Joachim
dc.contributor.authorBradley, Daniel G.
dc.contributor.authorCollins, Matthew J.
dc.date.accessioned2020-06-26T03:11:26Z
dc.date.available2020-06-26T03:11:26Z
dc.date.issued2020-06-25
dc.date.submitted2020-01-19
dc.identifier.otherpone-d-20-01701
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/307293
dc.descriptionFunder: FP7 Ideas: European Research Council; funder-id: http://dx.doi.org/10.13039/100011199; Grant(s): 295729
dc.description.abstractThe recovery and analysis of ancient DNA and protein from archaeological bone is time-consuming and expensive to carry out, while it involves the partial or complete destruction of valuable or rare specimens. The fields of palaeogenetic and palaeoproteomic research would benefit greatly from techniques that can assess the molecular quality prior to sampling. To be relevant, such screening methods should be effective, minimally-destructive, and rapid. This study reports results based on spectroscopic (Fourier-transform infrared spectroscopy in attenuated total reflectance [FTIR-ATR]; n = 266), palaeoproteomic (collagen content; n = 226), and palaeogenetic (endogenous DNA content; n = 88) techniques. We establish thresholds for three different FTIR indices, a) the infrared splitting factor [IRSF] that assesses relative changes in bioapatite crystals’ size and homogeneity; b) the carbonate-to-phosphate [C/P] ratio as a relative measure of carbonate content in bioapatite crystals; and c) the amide-to-phosphate ratio [Am/P] for assessing the relative organic content preserved in bone. These thresholds are both extremely reliable and easy to apply for the successful and rapid distinction between well- and poorly-preserved specimens. This is a milestone for choosing appropriate samples prior to genomic and collagen analyses, with important implications for biomolecular archaeology and palaeontology.
dc.languageen
dc.publisherPublic Library of Science
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectResearch Article
dc.subjectBiology and life sciences
dc.subjectEarth sciences
dc.subjectSocial sciences
dc.subjectEcology and environmental sciences
dc.titleScreening archaeological bone for palaeogenetic and palaeoproteomic studies
dc.typeArticle
dc.date.updated2020-06-26T03:11:25Z
prism.issueIdentifier6
prism.publicationNamePLOS ONE
prism.volume15
dc.identifier.doi10.17863/CAM.54390
dcterms.dateAccepted2020-06-09
rioxxterms.versionofrecord10.1371/journal.pone.0235146
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Petraglia, Michael D.
dc.contributor.orcidKontopoulos, Ioannis [0000-0001-5591-8917]
dc.contributor.orcidKreutzer, Susanne [0000-0001-6286-534X]
dc.contributor.orcidGehlen, Birgit [0000-0003-1345-8072]
dc.identifier.eissn1932-6203
pubs.funder-project-idAlexander S. Onassis Public Benefit Foundation (F ZL 047-1/2015-2016)
pubs.funder-project-idA.G. Leventis Foundation (PhD scholarship)
pubs.funder-project-idThe Greek Archaeological Committee UK (GACUK) (PhD scholarship)
pubs.funder-project-idLeverhulme Trust (PLP-2012-116)
pubs.funder-project-idDanmarks Grundforskningsfond (DNRF128)
pubs.funder-project-idVillum Fonden (10120)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)