Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment.
Ong Hui Chong, John
Levy, Galit Katarivas
Nature Publishing Group
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Ong Hui Chong, J., Zhao, J., Levy, G. K., Macdonald, J., Justin, A., & Markaki, A. (2020). Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment.. Scientific reports, 10 (1), 12429. https://doi.org/10.1038/s41598-020-69301-7
Albumin-based hydrogels are increasingly attractive in tissue engineering because they provide a xeno-free, biocompatible and potentially patient-specific platform for tissue engineering and drug delivery. The majority of research on albumin hydrogels has focused on bovine serum albumin (BSA), leaving human serum albumin (HSA) comparatively understudied. Different gelation methods are usually employed for HSA and BSA, and variations in the amino acid sequences of HSA and BSA exist; these account for differences in the hydrogel properties. Heat-induced gelation of aqueous HSA is the easiest method of synthesizing HSA hydrogels however hydrogel opacity and poor cell attachment limit their usefulness in downstream applications. Here, a solution to this problem is presented. Stable and translucent HSA hydrogels were created by controlled thermal gelation and the addition of sodium chloride. The resulting bio-inert hydrogel was then subjected to plasma treatment which functionalised its surface, enabling the attachment of basement membrane matrix (Geltrex). In vitro survival and proliferation studies of foetal human osteoblasts subsequently demonstrated good biocompatibility of functionalised albumin hydrogels compared to untreated samples. Thus, air plasma treatment enables functionalisation of inert heat-derived HSA hydrogels with extracellular matrix proteins and these may be used as a xeno-free platform for biomedical research or cell therapy.
Cell Line, Osteoblasts, Humans, Sodium Chloride, Extracellular Matrix Proteins, Biocompatible Materials, Hydrogels, Microscopy, Electron, Scanning, Tissue Engineering, Materials Testing, Cell Proliferation, Surface Properties, Hot Temperature, Plasma Gases, Serum Albumin, Human
JO is supported by the WD Armstrong Doctoral Fellowship, University of Cambridge and a Young NUS Fellowship, National University of Singapore (NUS). GKL is supported by the Blavatnik Family Foundation and the Reuben Foundation. JZ is supported by the Trinity College and the Cambridge Commonwealth, European and International Trust, University of Cambridge. AWJ is supported by the Isaac Newton Trust and the Rosetrees Trust (M787). JM is supported by a Engineering and Physical Sciences Research Council grant (EP/L016567/1), Pilkington NSG and the Worshipful Council of Engineers.
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External DOI: https://doi.org/10.1038/s41598-020-69301-7
This record's URL: https://www.repository.cam.ac.uk/handle/1810/307372
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