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dc.contributor.authorOng Hui Chong, Johnen
dc.contributor.authorZhao, Junzheen
dc.contributor.authorLevy, Galit Katarivasen
dc.contributor.authorMacdonald, Jamesen
dc.contributor.authorJustin, Alexanderen
dc.contributor.authorMarkaki, Athinaen
dc.date.accessioned2020-06-26T23:31:28Z
dc.date.available2020-06-26T23:31:28Z
dc.date.issued2020-07-24en
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/307372
dc.description.abstractAlbumin-based hydrogels are increasingly attractive in tissue engineering because they provide a xeno-free, biocompatible and potentially patient-specific platform for tissue engineering and drug delivery. The majority of research on albumin hydrogels has focused on bovine serum albumin (BSA), leaving human serum albumin (HSA) comparatively understudied. Different gelation methods are usually employed for HSA and BSA, and variations in the amino acid sequences of HSA and BSA exist; these account for differences in the hydrogel properties. Heat-induced gelation of aqueous HSA is the easiest method of synthesizing HSA hydrogels however hydrogel opacity and poor cell attachment limit their usefulness in downstream applications. Here, a solution to this problem is presented. Stable and translucent HSA hydrogels were created by controlled thermal gelation and the addition of sodium chloride. The resulting bio-inert hydrogel was then subjected to plasma treatment which functionalised its surface, enabling the attachment of basement membrane matrix (Geltrex). In vitro survival and proliferation studies of foetal human osteoblasts subsequently demonstrated good biocompatibility of functionalised albumin hydrogels compared to untreated samples. Thus, air plasma treatment enables functionalisation of inert heat-derived HSA hydrogels with extracellular matrix proteins and these may be used as a xeno-free platform for biomedical research or cell therapy.
dc.description.sponsorshipJO is supported by the WD Armstrong Doctoral Fellowship, University of Cambridge and a Young NUS Fellowship, National University of Singapore (NUS). GKL is supported by the Blavatnik Family Foundation and the Reuben Foundation. JZ is supported by the Trinity College and the Cambridge Commonwealth, European and International Trust, University of Cambridge. AWJ is supported by the Isaac Newton Trust and the Rosetrees Trust (M787). JM is supported by a Engineering and Physical Sciences Research Council grant (EP/L016567/1), Pilkington NSG and the Worshipful Council of Engineers.
dc.format.mediumElectronicen
dc.languageengen
dc.publisherNature Publishing Group
dc.rightsAll rights reserved
dc.subjectCell Lineen
dc.subjectOsteoblastsen
dc.subjectHumansen
dc.subjectSodium Chlorideen
dc.subjectExtracellular Matrix Proteinsen
dc.subjectBiocompatible Materialsen
dc.subjectHydrogelsen
dc.subjectMicroscopy, Electron, Scanningen
dc.subjectTissue Engineeringen
dc.subjectMaterials Testingen
dc.subjectCell Proliferationen
dc.subjectSurface Propertiesen
dc.subjectHot Temperatureen
dc.subjectPlasma Gasesen
dc.subjectSerum Albumin, Humanen
dc.titleFunctionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment.en
dc.typeArticle
prism.issueIdentifier1en
prism.publicationDate2020en
prism.publicationNameScientific reportsen
prism.startingPage12429
prism.volume10en
dc.identifier.doi10.17863/CAM.54462
dcterms.dateAccepted2020-06-22en
rioxxterms.versionofrecord10.1038/s41598-020-69301-7en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-07-24en
dc.contributor.orcidOng, John [0000-0001-5103-7311]
dc.contributor.orcidZhao, Junzhe [0000-0002-3224-9105]
dc.contributor.orcidMacdonald, James [0000-0002-5476-8600]
dc.contributor.orcidMarkaki, Athina [0000-0002-2265-1256]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (EP/L016567/1)
cam.orpheus.counter6*
rioxxterms.freetoread.startdate2023-06-26


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