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dc.contributor.authorUrbischek, Manuela
dc.contributor.authorRannikmae, Helena
dc.contributor.authorFoets, Thomas
dc.contributor.authorRavn, Katharina
dc.contributor.authorHyvönen, Marko
dc.contributor.authorde la Roche, Marc
dc.date.accessioned2020-07-09T15:12:37Z
dc.date.available2020-07-09T15:12:37Z
dc.date.issued2019-04-17
dc.date.submitted2018-10-05
dc.identifier.others41598-019-42604-0
dc.identifier.other42604
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/307800
dc.description.abstractAbstract: The media formulations necessary for deriving and sustaining organoids from epithelial tissues such as prostate, colon, gastric, liver, pancreas, and others have been established. Critical components of organoid media are a set of growth factors that include R-spondins and BMP signalling antagonists such as Noggin or Gremlin 1. Currently, the practical limitations for formulating organoid media of reproducible potency and larger-scale media production that have hampered further technological applications of organoid technology include: the cost of growth factors such as R-spondins and Gremlin 1/Noggin and their production as defined specific activities free of contaminants that may affect organoid growth. Here we report the production of highly pure recombinant Gremlin 1 and R-spondin 1 from bacterial expression for use in organoid media. We detail the workflow for Gremlin 1 and R-spondin 1 expression, purification, quantification of cellular activity, quality control and use in media formulated for culturing organoids derived from a number of tissues. The development of precisely formulated, cost-effective media of defined specific activity will engender the development of novel applications for organoid technology.
dc.languageen
dc.publisherNature Publishing Group UK
dc.subjectArticle
dc.subject/631/1647/767
dc.subject/631/80
dc.subject/13/100
dc.subject/13/106
dc.subjectarticle
dc.titleOrganoid culture media formulated with growth factors of defined cellular activity
dc.typeArticle
dc.date.updated2020-07-09T15:12:36Z
prism.issueIdentifier1
prism.publicationNameScientific Reports
prism.volume9
dc.identifier.doi10.17863/CAM.54892
dcterms.dateAccepted2019-04-03
rioxxterms.versionofrecord10.1038/s41598-019-42604-0
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn2045-2322


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