Within-host microevolution of Streptococcus pneumoniae is rapid and adaptive during natural colonisation
View / Open Files
Gladstone, Rebecca A.
Lo, Stephanie W.
Bancroft, Rowan E.
Klugman, Keith P.
Breiman, Robert F.
Barer, Michael R.
Adegbola, Richard A.
Nature Publishing Group UK
MetadataShow full item record
Chaguza, C., Senghore, M., Bojang, E., Gladstone, R. A., Lo, S. W., Tientcheu, P., Bancroft, R. E., et al. (2020). Within-host microevolution of Streptococcus pneumoniae is rapid and adaptive during natural colonisation. Nature Communications, 11 (1)https://doi.org/10.1038/s41467-020-17327-w
Funder: Bill and Melinda Gates Foundation (Bill & Melinda Gates Foundation)
Abstract: Genomic evolution, transmission and pathogenesis of Streptococcus pneumoniae, an opportunistic human-adapted pathogen, is driven principally by nasopharyngeal carriage. However, little is known about genomic changes during natural colonisation. Here, we use whole-genome sequencing to investigate within-host microevolution of naturally carried pneumococci in ninety-eight infants intensively sampled sequentially from birth until twelve months in a high-carriage African setting. We show that neutral evolution and nucleotide substitution rates up to forty-fold faster than observed over longer timescales in S. pneumoniae and other bacteria drives high within-host pneumococcal genetic diversity. Highly divergent co-existing strain variants emerge during colonisation episodes through real-time intra-host homologous recombination while the rest are co-transmitted or acquired independently during multiple colonisation episodes. Genic and intergenic parallel evolution occur particularly in antibiotic resistance, immune evasion and epithelial adhesion genes. Our findings suggest that within-host microevolution is rapid and adaptive during natural colonisation.
Article, /631/208/212/2304, /631/208/325/2482, /631/326/107, /692/308/3187, /45, /45/23, /13, /129, /141, /119, article
Bill and Melinda Gates Foundation (Bill & Melinda Gates Foundation) (OPP1034556, OPP1034556, OPP1034556)
External DOI: https://doi.org/10.1038/s41467-020-17327-w
This record's URL: https://www.repository.cam.ac.uk/handle/1810/307844