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dc.contributor.authorBatens, Maartenen
dc.contributor.authorShmool, Taliaen
dc.contributor.authorMassant, Janen
dc.contributor.authorZeitler, Axelen
dc.contributor.authorVan den Mooter, Guyen
dc.date.accessioned2020-07-10T23:30:28Z
dc.date.available2020-07-10T23:30:28Z
dc.date.issued2020-08en
dc.identifier.issn1463-9076
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/307859
dc.description.abstractThe β-relaxation associated with the sub-glass transition temperature (Tg,β ) is attributed to fast, localised molecular motions which can occur below the primary glass transition temperature (Tgα). Consistent with Tg,β being observed well-below storage temperatures, the β-relaxation associated motions have been hypothesised to influence protein stability in the solid state and could thus impact the quality of, e.g. protein powders for inhalation or reconstitution and injection. Why then do distinct solid state protein formulations with similar aggregation profiles after drying and immediate reconstitution, display different profiles when reconstituted following prolonged storage? Is the value of Tg,β , associated with the beta-relaxation process of the system, a reliable parameter for characterising the behaviour of proteins in the solid state? Bearing this in mind, in this work we further explore the different relaxation dynamics of glassy solid state monoclonal antibody formulations using terahertz time-domain spectroscopy and dynamical mechanical analysis. By conducting a 52-week stability study on a series of multi-component spray-dried formulations, an approach for characterising and analysing the solid-state dynamics and how these relate to protein stability is outlined.
dc.description.sponsorshipEPSRC
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherRoyal Society of Chemistry (RSC)
dc.rightsAll rights reserved
dc.rights.uri
dc.titleAdvancing predictions of protein stability in the solid state.en
dc.typeArticle
prism.endingPage17254
prism.issueIdentifier30en
prism.publicationDate2020en
prism.publicationNamePhysical chemistry chemical physics : PCCPen
prism.startingPage17247
prism.volume22en
dc.identifier.doi10.17863/CAM.54953
dcterms.dateAccepted2020-07-06en
rioxxterms.versionofrecord10.1039/d0cp00341gen
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-08en
dc.contributor.orcidShmool, Talia [0000-0002-0415-3050]
dc.contributor.orcidZeitler, Axel [0000-0002-4958-0582]
dc.identifier.eissn1463-9084
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (1198)
cam.orpheus.successMon Jul 13 08:26:41 BST 2020 - Embargo updated*
rioxxterms.freetoread.startdate2021-12-31


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