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dc.contributor.authorParaskevopoulou, Sofiaen
dc.contributor.authorPirzer, Fabianen
dc.contributor.authorGoldmann, Noraen
dc.contributor.authorSchmid, Julianen
dc.contributor.authorCorman, Victor Maxen
dc.contributor.authorGottula, Lina Theresaen
dc.contributor.authorSchroeder, Simonen
dc.contributor.authorRasche, Andreaen
dc.contributor.authorMuth, Doreenen
dc.contributor.authorDrexler, Jan Felixen
dc.contributor.authorHeni, Alexander Christophen
dc.contributor.authorEibner, Georg Joachimen
dc.contributor.authorPage, Rachel Aen
dc.contributor.authorJones, Terry Cen
dc.contributor.authorMüller, Marcel Aen
dc.contributor.authorSommer, Simoneen
dc.contributor.authorGlebe, Dieteren
dc.contributor.authorDrosten, Christianen
dc.date.accessioned2020-07-14T23:31:05Z
dc.date.available2020-07-14T23:31:05Z
dc.date.issued2020-07-10en
dc.identifier.issn0027-8424
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/307965
dc.description.abstract<jats:p>Hepatitis delta virus (HDV) is a human hepatitis-causing RNA virus, unrelated to any other taxonomic group of RNA viruses. Its occurrence as a satellite virus of hepatitis B virus (HBV) is a singular case in animal virology for which no consensus evolutionary explanation exists. Here we present a mammalian deltavirus that does not occur in humans, identified in the neotropical rodent species <jats:italic>Proechimys semispinosus</jats:italic>. The rodent deltavirus is highly distinct, showing a common ancestor with a recently described deltavirus in snakes. Reverse genetics based on a tandem minus-strand complementary DNA genome copy under the control of a cytomegalovirus (CMV) promoter confirms autonomous genome replication in transfected cells, with initiation of replication from the upstream genome copy. In contrast to HDV, a large delta antigen is not expressed and the farnesylation motif critical for HBV interaction is absent from a genome region that might correspond to a hypothetical rodent large delta antigen. Correspondingly, there is no evidence for coinfection with an HBV-related hepadnavirus based on virus detection and serology in any deltavirus-positive animal. No other coinfecting viruses were detected by RNA sequencing studies of 120 wild-caught animals that could serve as a potential helper virus. The presence of virus in blood and pronounced detection in reproductively active males suggest horizontal transmission linked to competitive behavior. Our study establishes a nonhuman, mammalian deltavirus that occurs as a horizontally transmitted infection, is potentially cleared by immune response, is not focused in the liver, and possibly does not require helper virus coinfection.</jats:p>
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherProceedings of the National Academy of Sciences
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCell Line, Tumoren
dc.subjectAnimalsen
dc.subjectHumansen
dc.subjectRodentiaen
dc.subjectHepatitis Delta Virusen
dc.subjectHepadnaviridaeen
dc.subjectHepadnaviridae Infectionsen
dc.subjectHepatitis Den
dc.subjectRodent Diseasesen
dc.subjectGenomicsen
dc.subjectPhylogenyen
dc.subjectGenome, Viralen
dc.subjectCoinfectionen
dc.titleMammalian deltavirus without hepadnavirus coinfection in the neotropical rodent <i>Proechimys semispinosus</i>.en
dc.typeArticle
prism.endingPage17983
prism.issueIdentifier30en
prism.publicationDate2020en
prism.publicationNameProceedings of the National Academy of Sciences of the United States of Americaen
prism.startingPage17977
prism.volume117en
dc.identifier.doi10.17863/CAM.55057
dcterms.dateAccepted2020-06-03en
rioxxterms.versionofrecord10.1073/pnas.2006750117en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-07-10en
dc.contributor.orcidParaskevopoulou, Sofia [0000-0003-2608-2596]
dc.contributor.orcidPirzer, Fabian [0000-0001-8525-8593]
dc.contributor.orcidSchmid, Julian [0000-0003-1775-6958]
dc.contributor.orcidCorman, Victor Max [0000-0002-3605-0136]
dc.contributor.orcidGottula, Lina Theresa [0000-0001-5518-3957]
dc.contributor.orcidSchroeder, Simon [0000-0003-2085-767X]
dc.contributor.orcidRasche, Andrea [0000-0001-6693-1180]
dc.contributor.orcidMuth, Doreen [0000-0001-9992-5393]
dc.contributor.orcidJones, Terry C [0000-0003-1120-9531]
dc.contributor.orcidSommer, Simone [0000-0002-5148-8136]
dc.identifier.eissn1091-6490
rioxxterms.typeJournal Article/Reviewen


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International