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dc.contributor.authorPokhare, Somnath Sen
dc.contributor.authorThorpe, Peteren
dc.contributor.authorHedley, Peteen
dc.contributor.authorMorris, Jenniferen
dc.contributor.authorHabash, Samer Sen
dc.contributor.authorElashry, Abdelnaseren
dc.contributor.authorEves-Van Den Akker, Sebastianen
dc.contributor.authorGrundler, Florian MWen
dc.contributor.authorJones, John Ten
dc.date.accessioned2020-07-16T00:25:25Z
dc.date.available2020-07-16T00:25:25Z
dc.date.issued2020-08en
dc.identifier.issn0960-7412
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/308002
dc.description.abstractInteractions between plant-parasitic nematodes and their hosts are mediated by effectors; secreted proteins that manipulate the plant to the benefit of the pathogen. To understand the role of effectors in host adaptation in nematodes we analysed the transcriptome of Heterodera sacchari, a cyst nematode parasite of rice and sugarcane. A multi-gene phylogenetic analysis showed that H. sacchari and the cereal cyst nematode Heterodera aveneae share a common evolutionary origin and that they evolved to parasitise monocot plants from a common dicot-parasitic ancestor. We compared the effector repertoires of H. sacchari with those of the dicot parasites H. glycines and Globodera rostochiensis to understand the consequences of this transition. While, in general, effector repertoires are similar between the species, comparing effectors and non-effectors of H. sacchari and G. rostochiensis shows that effectors have accumulated more mutations than non-effectors. Although most effectors show conserved spatiotemporal expression profiles and likely function, some H. sacchari effectors are adapted to monocots. This is exemplified by the plant-peptide hormone mimics, the CLAVATA3/EMBRYO SURROUNDING REGION-like (CLE) effectors. Peptide hormones encoded by H. sacchari CLE effectors are more similar to those from rice than those from other plants, or those from other plant-parasitic nematodes. We experimentally validate the functional significance of these observations by demonstrating that CLE peptides encoded by H. sacchari induce a short root phenotype in rice, whereas those from a related dicot parasite do not. These data provide a functional example of effector evolution that co-occurred with the transition from dicot-parasitic to a monocot-parasitic lifestyle.
dc.description.sponsorshipBBSRC
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherWiley-Blackwell
dc.rightsAll rights reserved
dc.subjectAnimalsen
dc.subjectTylenchoideaen
dc.subjectPeptide Hormonesen
dc.subjectHelminth Proteinsen
dc.subjectPlant Diseasesen
dc.subjectHost-Parasite Interactionsen
dc.subjectTranscriptomeen
dc.titleSignatures of adaptation to a monocot host in the plant-parasitic cyst nematode Heterodera sacchari.en
dc.typeArticle
prism.endingPage1274
prism.issueIdentifier4en
prism.publicationDate2020en
prism.publicationNameThe Plant journal : for cell and molecular biologyen
prism.startingPage1263
prism.volume103en
dc.identifier.doi10.17863/CAM.55098
dcterms.dateAccepted2020-06-18en
rioxxterms.versionofrecord10.1111/tpj.14910en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-08en
dc.contributor.orcidHedley, Pete [0000-0003-0866-324X]
dc.contributor.orcidEves-Van Den Akker, Sebastian [0000-0002-8833-9679]
dc.contributor.orcidJones, John T [0000-0003-2074-0551]
dc.identifier.eissn1365-313X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/R011311/1)
pubs.funder-project-idBBSRC (BB/S006397/1)
cam.orpheus.successTue Sep 01 14:00:10 BST 2020 - Embargo updated*
rioxxterms.freetoread.startdate2021-08-31


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