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Innovations, challenges, and minimal information for standardization of humanized mice.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Schuringa, Jan Jacob  ORCID logo  https://orcid.org/0000-0001-8452-8555
Bissig, Karl-Dimiter 
Soper, Brian 

Abstract

Mice xenotransplanted with human cells and/or expressing human gene products (also known as "humanized mice") recapitulate the human evolutionary specialization and diversity of genotypic and phenotypic traits. These models can provide a relevant in vivo context for understanding of human-specific physiology and pathologies. Humanized mice have advanced toward mainstream preclinical models and are now at the forefront of biomedical research. Here, we considered innovations and challenges regarding the reconstitution of human immunity and human tissues, modeling of human infections and cancer, and the use of humanized mice for testing drugs or regenerative therapy products. As the number of publications exploring different facets of humanized mouse models has steadily increased in past years, it is becoming evident that standardized reporting is needed in the field. Therefore, an international community-driven resource called "Minimal Information for Standardization of Humanized Mice" (MISHUM) has been created for the purpose of enhancing rigor and reproducibility of studies in the field. Within MISHUM, we propose comprehensive guidelines for reporting critical information generated using humanized mice.

Description

Keywords

PDX, humanized mice, immuno-oncology, infections, regenerative medicine, Animals, Disease Models, Animal, Guidelines as Topic, Heterografts, Humans, Mice, Mice, SCID, Neoplasms, Reproducibility of Results

Journal Title

EMBO Mol Med

Conference Name

Journal ISSN

1757-4676
1757-4684

Volume Title

12

Publisher

Springer Science and Business Media LLC