Mapping RNA-Chromatin Interactions In Vivo with RNA-DamID.
Cheetham, Seth W
Methods in Molecular Biology
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Cheetham, S. W., & Brand, A. (2020). Mapping RNA-Chromatin Interactions In Vivo with RNA-DamID.. Methods in Molecular Biology, 2161 255-264. https://doi.org/10.1007/978-1-0716-0680-3_18
Long-noncoding RNAs (lncRNAs) are emerging as regulators of development and disease. lncRNAs are expressed in exquisitely precise expression patterns in vivo and many interact with chromatin to regulate gene expression. However, the limited sensitivity of RNA-purification techniques has precluded the identification of genomic targets of cell-type specific lncRNAs. RNA-DamID is a powerful new approach to understand the mechanisms by which lncRNAs act in vivo. RNA-DamID is highly sensitive and accurate, and can resolve cell-type-specific chromatin binding patterns without cell isolation. The determinants of RNA-chromatin interactions can be identified with RNA-DamID by analyzing RNA and protein cofactor mutants. Here we describe how to implement RNA-DamID and the design considerations to take into account to accurately identify lncRNA-chromatin interactions in vivo.
Wellcome Trust (092096/Z/10/Z)
Cancer Research UK (A14492)
WELLCOME TRUST (103792/Z/14/Z)
Royal Society (RP150061)
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External DOI: https://doi.org/10.1007/978-1-0716-0680-3_18
This record's URL: https://www.repository.cam.ac.uk/handle/1810/308706
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