A decade of advances in transposon-insertion sequencing.
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Authors
Cain, Amy K
Barquist, Lars
Goodman, Andrew L
Paulsen, Ian T
van Opijnen, Tim
Publication Date
2020-06-12Journal Title
Nature Reviews Genetics
ISSN
1471-0056
Publisher
Springer Nature
Language
eng
Type
Article
This Version
AM
Metadata
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Cain, A. K., Barquist, L., Goodman, A. L., Paulsen, I. T., Parkhill, J., & van Opijnen, T. (2020). A decade of advances in transposon-insertion sequencing.. Nature Reviews Genetics https://doi.org/10.1038/s41576-020-0244-x
Abstract
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which combine genome-wide transposon mutagenesis with high-throughput sequencing to estimate the fitness contribution or essentiality of each genetic component in a bacterial genome. Four TIS variations were published in 2009: transposon sequencing (Tn-Seq), transposon-directed insertion site sequencing (TraDIS), insertion sequencing (INSeq) and high-throughput insertion tracking by deep sequencing (HITS). TIS has since become an important tool for molecular microbiologists, being one of the few genome-wide techniques that directly links phenotype to genotype and ultimately can assign gene function. In this Review, we discuss the recent applications of TIS to answer overarching biological questions. We explore emerging and multidisciplinary methods that build on TIS, with an eye towards future applications.
Sponsorship
Medical Research Council (G1100100)
Identifiers
External DOI: https://doi.org/10.1038/s41576-020-0244-x
This record's URL: https://www.repository.cam.ac.uk/handle/1810/308771
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