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dc.contributor.authorReyes, Aurelio
dc.contributor.authorFavia, Paola
dc.contributor.authorVidoni, Sara
dc.contributor.authorPetruzzella, Vittoria
dc.contributor.authorZeviani, Massimo
dc.date.accessioned2020-08-13T03:11:04Z
dc.date.available2020-08-13T03:11:04Z
dc.date.issued2020-07-31
dc.date.submitted2020-02-06
dc.identifier.issn1553-7390
dc.identifier.otherpgenetics-d-20-00176
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/309108
dc.description.abstractMitochondrial translation defects can be due to mutations affecting mitochondrial- or nuclear-encoded components. The number of known nuclear genes involved in mitochondrial translation has significantly increased in the past years. RCC1L (WBSCR16), a putative GDP/GTP exchange factor, has recently been described to interact with the mitochondrial large ribosomal subunit. In humans, three different RCC1L isoforms have been identified that originate from alternative splicing but share the same N-terminus, RCC1LV1, RCC1LV2 and RCC1LV3. All three isoforms were exclusively localized to mitochondria, interacted with its inner membrane and could associate with homopolymeric oligos to different extent. Mitochondrial immunoprecipitation experiments showed that RCC1LV1 and RCC1LV3 associated with the mitochondrial large and small ribosomal subunit, respectively, while no significant association was observed for RCC1LV2. Overexpression and silencing of RCC1LV1 or RCC1LV3 led to mitoribosome biogenesis defects that resulted in decreased translation. Indeed, significant changes in steady-state levels and distribution on isokinetic sucrose gradients were detected not only for mitoribosome proteins but also for GTPases, (GTPBP10, ERAL1 and C4orf14), and pseudouridylation proteins, (TRUB2, RPUSD3 and RPUSD4). All in all, our data suggest that RCC1L is essential for mitochondrial function and that the coordination of at least two isoforms is essential for proper ribosomal assembly.
dc.languageen
dc.publisherPublic Library of Science
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectResearch Article
dc.subjectBiology and life sciences
dc.subjectResearch and analysis methods
dc.titleRCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases
dc.typeArticle
dc.date.updated2020-08-13T03:11:03Z
prism.issueIdentifier7
prism.publicationNamePLOS Genetics
prism.volume16
dc.identifier.doi10.17863/CAM.56202
dcterms.dateAccepted2020-06-10
rioxxterms.versionofrecord10.1371/journal.pgen.1008923
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Filipovska, Aleksandra
dc.contributor.orcidReyes, Aurelio [0000-0003-2876-2202]
dc.contributor.orcidFavia, Paola [0000-0002-1451-9124]
dc.contributor.orcidVidoni, Sara [0000-0001-5071-0738]
dc.contributor.orcidPetruzzella, Vittoria [0000-0001-8771-6033]
dc.contributor.orcidZeviani, Massimo [0000-0002-9067-5508]
dc.identifier.eissn1553-7404


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)