TBR2 coordinates neurogenesis expansion and precise microcircuit organization via Protocadherin 19 in the mammalian cortex
Authors
Lv, Xiaohui
Ren, Si-Qiang
Zhang, Xin-Jun
Shen, Zhongfu
Ghosh, Tanay
Xianyu, Anjin
Gao, Peng
Li, Zhizhong
Lin, Susan
Yu, Yang
Zhang, Qiangqiang
Groszer, Matthias
Shi, Song-Hai
Publication Date
2019-09-02Journal Title
Nature Communications
Publisher
Nature Publishing Group UK
Volume
10
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Lv, X., Ren, S., Zhang, X., Shen, Z., Ghosh, T., Xianyu, A., Gao, P., et al. (2019). TBR2 coordinates neurogenesis expansion and precise microcircuit organization via Protocadherin 19 in the mammalian cortex. Nature Communications, 10 (1)https://doi.org/10.1038/s41467-019-11854-x
Abstract
Abstract: Cerebral cortex expansion is a hallmark of mammalian brain evolution; yet, how increased neurogenesis is coordinated with structural and functional development remains largely unclear. The T-box protein TBR2/EOMES is preferentially enriched in intermediate progenitors and supports cortical neurogenesis expansion. Here we show that TBR2 regulates fine-scale spatial and circuit organization of excitatory neurons in addition to enhancing neurogenesis in the mouse cortex. TBR2 removal leads to a significant reduction in neuronal, but not glial, output of individual radial glial progenitors as revealed by mosaic analysis with double markers. Moreover, in the absence of TBR2, clonally related excitatory neurons become more laterally dispersed and their preferential synapse development is impaired. Interestingly, TBR2 directly regulates the expression of Protocadherin 19 (PCDH19), and simultaneous PCDH19 expression rescues neurogenesis and neuronal organization defects caused by TBR2 removal. Together, these results suggest that TBR2 coordinates neurogenesis expansion and precise microcircuit assembly via PCDH19 in the mammalian cortex.
Keywords
Article, /631/136/368, /631/136/532/2182, /13/51, /13/106, /14/19, /14/32, /38/61, article
Identifiers
s41467-019-11854-x, 11854
External DOI: https://doi.org/10.1038/s41467-019-11854-x
This record's URL: https://www.repository.cam.ac.uk/handle/1810/309793
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/