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dc.contributor.authorLager, Susanneen
dc.contributor.authorSovio, Ullaen
dc.contributor.authorEddershaw, Elizabethen
dc.contributor.authorvan der Linden, Margaretha Wen
dc.contributor.authorYazar, Cansuen
dc.contributor.authorCook, Emmaen
dc.contributor.authorHapperfield, Lisaen
dc.contributor.authorJessop, Flora Aen
dc.contributor.authorSebire, Neil Jen
dc.contributor.authorCharnock-Jones, Stephenen
dc.contributor.authorSmith, Gordonen
dc.date.accessioned2020-09-11T23:31:25Z
dc.date.available2020-09-11T23:31:25Z
dc.date.issued2020-12en
dc.identifier.issn0022-3751
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/310199
dc.description.abstractAbstract Fetal growth restriction (FGR) and pre-eclampsia are severe, adverse pregnancy outcomes. Alterations in placental histology are frequently reported in these pregnancy complications and are often based upon scoring by pathologists. However, many alterations are also observed in placenta from uncomplicated pregnancies. Moreover, knowledge of disease state may bias assessment. We sought to perform an objective comparison of placental microscopic appearance in normal and complicated pregnancies. Placental villous tissue (n=823) and edge biopsies (n=488) from 871 individual, singleton pregnancies were collected after delivery. Cases of small-for-gestational-age (SGA) or pre-eclampsia were matched with healthy controls. A subset of the SGA cases displayed signs of FGR. Cases of pre-term delivery were also included. Tissue sections were stained with H&E or antibodies for CD8, CD14, CD31, CD79α, elastase. Images were scored by two experienced pathologists for pathological features or analysed by image analysis and stereology. Analyses were performed blind to case-control status and gestational age. Volume fraction of T-cells increased in placentas from pregnancies complicated by pre-eclampsia (adjusted odds ratio (aOR) 1.46, 95% CI 1.12-1.90) and FGR (aOR 1.64, 95% CI 1.11-2.43), whereas B-cells only increased in FGR (aOR 1.65, 95% CI 1.05-2.60). Pathological abnormalities in villous tissue were reported in 21.4% (88/411) of complicated pregnancies and 14.3% (52/363) of controls (OR 1.62, 95% CI 1.12-2.37). There were no differences in the fractions of endothelial cells, fibrin deposition, macrophages, and neutrophils when comparing normal and complicated pregnancies. In conclusion, FGR and pre-eclampsia are associated with T-cell infiltration of the placenta and placental pathological abnormalities.
dc.description.sponsorshipThis study was supported by grants from the Medical Research Council (United Kingdom; MR/K021133/1) and the National Institute for Health Research (NIHR) Cambridge Biomedical Research Centre (Women’s Health theme). The funding sources had no involvement in study design, data collection, analysis or interpretation, manuscript preparation or deciding to submit the article for publication.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.rightsAll rights reserved
dc.rights.uri
dc.titleAbnormal placental CD8<sup>+</sup> T-cell infiltration is a feature of fetal growth restriction and pre-eclampsia.en
dc.typeArticle
prism.endingPage5571
prism.issueIdentifier23en
prism.publicationDate2020en
prism.publicationNameThe Journal of physiologyen
prism.startingPage5555
prism.volume598en
dc.identifier.doi10.17863/CAM.57285
dcterms.dateAccepted2020-08-28en
rioxxterms.versionofrecord10.1113/jp279532en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-12en
dc.contributor.orcidLager, Susanne [0000-0003-3556-065X]
dc.contributor.orcidSovio, Ulla [0000-0002-0799-1105]
dc.contributor.orcidYazar, Cansu [0000-0003-2660-0111]
dc.contributor.orcidCook, Emma [0000-0002-6142-4443]
dc.contributor.orcidSebire, Neil J [0000-0001-5348-9063]
dc.contributor.orcidCharnock-Jones, David [0000-0002-2936-4890]
dc.contributor.orcidSmith, Gordon [0000-0003-2124-0997]
dc.identifier.eissn1469-7793
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MR/K021133/1)
pubs.funder-project-idCambridge University Hospitals NHS Foundation Trust (CUH) (146281)
cam.orpheus.successMon Sep 14 09:22:24 BST 2020 - Embargo updated*
rioxxterms.freetoread.startdate2021-09-04


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