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Modifying bacterial flagellin to evade Nod-like Receptor CARD 4 recognition enhances protective immunity against Salmonella.

Accepted version
Peer-reviewed

Type

Article

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Authors

Tourlomousis, Panagiotis  ORCID logo  https://orcid.org/0000-0002-6152-8066
Bittante, Alessandra S 
Hopkins, Lee J 
Webster, Steven J 

Abstract

Pattern recognition receptors (PRRs) expressed in antigen-presenting cells are thought to shape pathogen-specific immunity by inducing secretion of costimulatory cytokines during T-cell activation, yet data to support this notion in vivo are scarce. Here, we show that the cytosolic PRR Nod-like Receptor CARD 4 (NLRC4) suppresses, rather than facilitates, effector and memory CD4+ T-cell responses against Salmonella in mice. NLRC4 negatively regulates immunological memory by preventing delayed activation of the cytosolic PRR NLR pyrin domain 3 (NLRP3) that would otherwise amplify the production of cytokines important for the generation of Th1 immunity such as intereukin-18. Consistent with a role for NLRC4 in memory immunity, primary challenge with Salmonella expressing flagellin modified to largely evade NLRC4 recognition notably increases protection against lethal rechallenge. This finding suggests flagellin modification to reduce NLRC4 activation enhances protective immunity, which could have important implications for vaccine development against flagellated microbial pathogens.

Description

Keywords

Animals, Apoptosis Regulatory Proteins, Calcium-Binding Proteins, Female, Flagellin, Host-Pathogen Interactions, Humans, Immunity, Innate, Interleukin-18, Macrophages, Male, Mice, Mice, Inbred C57BL, NLR Family, Pyrin Domain-Containing 3 Protein, Salmonella Infections, Salmonella typhimurium, T-Lymphocytes, Th1 Cells

Journal Title

Nat Microbiol

Conference Name

Journal ISSN

2058-5276
2058-5276

Volume Title

5

Publisher

Springer Science and Business Media LLC

Rights

All rights reserved
Sponsorship
Wellcome Trust (108045/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/H003916/1)
Biotechnology and Biological Sciences Research Council (BB/K006436/1)
Wellcome Trust
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