Show simple item record

dc.contributor.authorMatos, Maria J
dc.contributor.authorBrown, Libby
dc.contributor.authorBernardim, Barbara
dc.contributor.authorGuerreiro, Ana
dc.contributor.authorJiménez-Osés, Gonzalo
dc.contributor.authorBernardes, Gonçalo JL
dc.date.accessioned2020-09-25T23:30:37Z
dc.date.available2020-09-25T23:30:37Z
dc.date.issued2020-11-15
dc.identifier.issn0968-0896
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/310748
dc.description.abstractMethods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPeptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
dc.subjectLysine
dc.subjectAlbumins
dc.subjectAntibodies
dc.subjectMolecular Structure
dc.titleSequential dual site-selective protein labelling enabled by lysine modification.
dc.typeArticle
prism.issueIdentifier22
prism.publicationDate2020
prism.publicationNameBioorg Med Chem
prism.startingPage115783
prism.volume28
dc.identifier.doi10.17863/CAM.57837
dcterms.dateAccepted2020-09-16
rioxxterms.versionofrecord10.1016/j.bmc.2020.115783
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-11
dc.contributor.orcidBernardim de Souza, Barbara [0000-0002-0554-2728]
dc.contributor.orcidLopes Bernardes, Goncalo [0000-0001-6594-8917]
dc.identifier.eissn1464-3391
rioxxterms.typeJournal Article/Review
pubs.funder-project-idRoyal Society (URF\R\180019)
cam.issuedOnline2020-09-24
cam.orpheus.successTue Oct 06 10:23:57 BST 2020 - Embargo updated
rioxxterms.freetoread.startdate2021-09-30


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International