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dc.contributor.authorO'Neill, John
dc.contributor.authorHoyle, Nathaniel P
dc.contributor.authorRobertson, J Brian
dc.contributor.authorEdgar, Rachel S
dc.contributor.authorBeale, Andrew D
dc.contributor.authorPeak-Chew, Sew Y
dc.contributor.authorDay, Jason
dc.contributor.authorCosta, Ana SH
dc.contributor.authorFrezza, Christian
dc.contributor.authorCauston, Helen C
dc.date.accessioned2020-10-05T23:31:25Z
dc.date.available2020-10-05T23:31:25Z
dc.date.issued2020-09-17
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/311112
dc.description.abstractYeast physiology is temporally regulated, this becomes apparent under nutrient-limited conditions and results in respiratory oscillations (YROs). YROs share features with circadian rhythms and interact with, but are independent of, the cell division cycle. Here, we show that YROs minimise energy expenditure by restricting protein synthesis until sufficient resources are stored, while maintaining osmotic homeostasis and protein quality control. Although nutrient supply is constant, cells sequester and store metabolic resources via increased transport, autophagy and biomolecular condensation. Replete stores trigger increased H+ export which stimulates TORC1 and liberates proteasomes, ribosomes, chaperones and metabolic enzymes from non-membrane bound compartments. This facilitates translational bursting, liquidation of storage carbohydrates, increased ATP turnover, and the export of osmolytes. We propose that dynamic regulation of ion transport and metabolic plasticity are required to maintain osmotic and protein homeostasis during remodelling of eukaryotic proteomes, and that bioenergetic constraints selected for temporal organisation that promotes oscillatory behaviour.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectRibosomes
dc.subjectEukaryotic Cells
dc.subjectYeasts
dc.subjectOxygen
dc.subjectGlycogen
dc.subjectIonomycin
dc.subjectMolecular Chaperones
dc.subjectProteome
dc.subjectBioreactors
dc.subjectProteomics
dc.subjectProtein Biosynthesis
dc.subjectProtein Processing, Post-Translational
dc.subjectEnergy Metabolism
dc.subjectCircadian Rhythm
dc.subjectHeat-Shock Response
dc.subjectOsmolar Concentration
dc.subjectOsmotic Pressure
dc.subjectAutophagy
dc.subjectMetabolomics
dc.subjectProteostasis
dc.subjectMechanistic Target of Rapamycin Complex 1
dc.titleEukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2020
prism.publicationNameNat Commun
prism.startingPage4706
prism.volume11
dc.identifier.doi10.17863/CAM.58201
dcterms.dateAccepted2020-08-13
rioxxterms.versionofrecord10.1038/s41467-020-18330-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-09-17
dc.contributor.orcidO'Neill, John [0000-0003-2204-6096]
dc.contributor.orcidHoyle, Nathaniel P [0000-0002-3250-0494]
dc.contributor.orcidBeale, Andrew D [0000-0002-2051-0919]
dc.contributor.orcidPeak-Chew, Sew Y [0000-0002-7602-6384]
dc.contributor.orcidCosta, Ana SH [0000-0001-8932-6370]
dc.contributor.orcidFrezza, Christian [0000-0002-3293-7397]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MC_UU_12022/6)
cam.issuedOnline2020-09-17


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International