Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction
Authors
Ahmed, Ibrahim
Tucci, Felicia A.
Aflalo, Aure
Smith, Kenneth G. C.
Bashford-Rogers, Rachael J. M.
Publication Date
2020-06-29Journal Title
Scientific Reports
Publisher
Nature Publishing Group UK
Volume
10
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Ahmed, I., Tucci, F. A., Aflalo, A., Smith, K. G. C., & Bashford-Rogers, R. J. M. (2020). Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction. Scientific Reports, 10 (1)https://doi.org/10.1038/s41598-020-67290-1
Abstract
Abstract: The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces the inflation/deflation of DNA variant proportions due to PCR amplification biases and sequencing errors. This method was applied to immune receptor sequencing, where it significantly improves the quality and estimation of diversity of the resulting library.
Keywords
Article, /631/61, /631/250/2152/2497, /631/208/727, /631/208/737, /631/208/514, article
Identifiers
s41598-020-67290-1, 67290
External DOI: https://doi.org/10.1038/s41598-020-67290-1
This record's URL: https://www.repository.cam.ac.uk/handle/1810/311189
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/