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dc.contributor.authorAhmed, Ibrahim
dc.contributor.authorTucci, Felicia A.
dc.contributor.authorAflalo, Aure
dc.contributor.authorSmith, Kenneth G. C.
dc.contributor.authorBashford-Rogers, Rachael J. M.
dc.date.accessioned2020-10-07T16:19:01Z
dc.date.available2020-10-07T16:19:01Z
dc.date.issued2020-06-29
dc.date.submitted2019-11-08
dc.identifier.others41598-020-67290-1
dc.identifier.other67290
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/311189
dc.description.abstractAbstract: The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces the inflation/deflation of DNA variant proportions due to PCR amplification biases and sequencing errors. This method was applied to immune receptor sequencing, where it significantly improves the quality and estimation of diversity of the resulting library.
dc.languageen
dc.publisherNature Publishing Group UK
dc.subjectArticle
dc.subject/631/61
dc.subject/631/250/2152/2497
dc.subject/631/208/727
dc.subject/631/208/737
dc.subject/631/208/514
dc.subjectarticle
dc.titleUltrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction
dc.typeArticle
dc.date.updated2020-10-07T16:19:00Z
prism.issueIdentifier1
prism.publicationNameScientific Reports
prism.volume10
dc.identifier.doi10.17863/CAM.58281
dcterms.dateAccepted2020-06-01
rioxxterms.versionofrecord10.1038/s41598-020-67290-1
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn2045-2322


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