Chromosome engineering in zygotes with CRISPR/Cas9.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Boroviak, Katharina
Doe, Brendan
Banerjee, Ruby
Yang, Fengtang
Bradley, Allan https://orcid.org/0000-0002-2349-8839
Abstract
Deletions, duplications, and inversions of large genomic regions covering several genes are an important class of disease causing variants in humans. Modeling these structural variants in mice requires multistep processes in ES cells, which has limited their availability. Mutant mice containing small insertions, deletions, and single nucleotide polymorphisms can be reliably generated using CRISPR/Cas9 directly in mouse zygotes. Large structural variants can be generated using CRISPR/Cas9 in ES cells, but it has not been possible to generate these directly in zygotes. We now demonstrate the direct generation of deletions, duplications and inversions of up to one million base pairs by zygote injection.
Description
Keywords
CRISPR/Cas9, large structural variants, zygote injection, Animals, Base Sequence, CRISPR-Cas Systems, Chromosome Duplication, Chromosome Inversion, Chromosomes, DNA, Feasibility Studies, Female, Genetic Engineering, Male, Mice, Mice, Inbred C57BL, Molecular Sequence Data
Journal Title
Genesis
Conference Name
Journal ISSN
1526-954X
1526-968X
1526-968X
Volume Title
54
Publisher
Wiley