Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species.
Published version
Peer-reviewed
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Authors
Vetukuri, Ramesh R
Kalyandurg, Pruthvi B
Saripella, Ganapathi Varma
Sen, Diya
Gil, Jose Fernando
Abstract
Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5'-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.
Description
Keywords
Carlavirus, Chrysanthemum, Cytoplasm, MicroRNAs, RNA Interference, RNA, Plant, RNA, Small Interfering
Journal Title
Arch Virol
Conference Name
Journal ISSN
0304-8608
1432-8798
1432-8798
Volume Title
165
Publisher
Springer Science and Business Media LLC