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Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers.

Published version
Peer-reviewed

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Authors

Kolesnikova, Larissa 
Koehler, Alexander 

Abstract

Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.

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Keywords

Ebola virus, Marburg virus, infectious disease, matrix protein, microbiology, molecular biophysics, structural biology, Cell Membrane, Ebolavirus, Marburgvirus, Protein Multimerization, Viral Matrix Proteins

Journal Title

Elife

Conference Name

Journal ISSN

2050-084X
2050-084X

Volume Title

9

Publisher

eLife Sciences Publications, Ltd
Sponsorship
Medical Research Council (MC_UP_1201/16)
H2020 European Research Council (ERC-CoG-648432)
Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 1021)