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dc.contributor.authorNavarro, Isabela Cunhaen
dc.contributor.authorTuorto, Francescaen
dc.contributor.authorJordan, Daviden
dc.contributor.authorLegrand, Carineen
dc.contributor.authorPrice, Jonathanen
dc.contributor.authorBraukmann, Fabianen
dc.contributor.authorHendrick, Alanen
dc.contributor.authorAkay, Alperen
dc.contributor.authorKotter, Annikaen
dc.contributor.authorHelm, Marken
dc.contributor.authorLyko, Franken
dc.contributor.authorMiska, Ericen
dc.date.accessioned2020-11-13T00:30:34Z
dc.date.available2020-11-13T00:30:34Z
dc.date.issued2021-03en
dc.identifier.issn0261-4189
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/312830
dc.description.abstractABSTRACT Methylation of carbon-5 of cytosines (m 5 C) is a post-transcriptional nucleotide modification of RNA found in all kingdoms of life. While individual m 5 C-methyltransferases have been studied, the impact of the global cytosine-5 methylome on development, homeostasis and stress remains unknown. Here, using Caenorhabditis elegans , we generated the first organism devoid of m 5 C in RNA, demonstrating that this modification is non-essential. We determined the localisation and enzymatic specificity of m 5 C sites in RNA in vivo and showed that animals devoid of m 5 C are sensitive to temperature stress. At the molecular level, we showed that loss of m 5 C specifically impacts decoding of leucine and proline thus reducing the translation efficiency of transcripts enriched in these amino acids. Finally, we found translation of leucine UUG codons to be the most strongly affected upon heat shock, suggesting a role of m 5 C tRNA wobble methylation in the adaptation to heat stress.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherEMBO Press
dc.rightsAll rights reserved
dc.titleTranslational adaptation to heat stress is mediated by RNA 5-methylcytosine in Caenorhabditis elegans.en
dc.typeArticle
prism.issueIdentifier6en
prism.publicationDate2021en
prism.publicationNameThe EMBO journalen
prism.startingPagee105496
prism.volume40en
dc.identifier.doi10.17863/CAM.59928
dcterms.dateAccepted2020-11-10en
rioxxterms.versionofrecord10.15252/embj.2020105496en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2021-03en
dc.contributor.orcidNavarro, Isabela Cunha [0000-0002-6844-2361]
dc.contributor.orcidTuorto, Francesca [0000-0003-1625-1181]
dc.contributor.orcidLegrand, Carine [0000-0002-0992-3886]
dc.contributor.orcidPrice, Jonathan [0000-0001-6554-5667]
dc.contributor.orcidBraukmann, Fabian [0000-0003-2660-450X]
dc.contributor.orcidHendrick, Alan [0000-0002-8604-0462]
dc.contributor.orcidAkay, Alper [0000-0001-6825-4443]
dc.contributor.orcidMiska, Eric [0000-0002-4450-576X]
dc.identifier.eissn1460-2075
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWELLCOME TRUST (104640/Z/14/Z)
pubs.funder-project-idCancer Research UK (A27826)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idWellcome Trust (203144/Z/16/Z)
pubs.funder-project-idCancer Research UK (C6946/A24843)
cam.orpheus.successMon Dec 14 07:30:51 GMT 2020 - Embargo updated*
cam.orpheus.counter7*
rioxxterms.freetoread.startdate2021-06-07


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