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dc.contributor.authorOlivares, Gen
dc.contributor.authorHermes, Den
dc.contributor.authorManzanilla, Een
dc.contributor.authorHughes, Ken
dc.contributor.authorClarke, Len
dc.contributor.authorWarland, Jamesen
dc.date.accessioned2020-11-14T00:30:16Z
dc.date.available2020-11-14T00:30:16Z
dc.date.issued2020-12-04en
dc.identifier.issn0022-4510
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/312882
dc.description.abstractStructured summary: o Objective To determine the usefulness of bacteriology swabs as a storage method of canine urine samples and the effect on quantitative bacterial culture (QBC) o Material and methods Two hundred fourteen canine urine samples were collected by cystocentesis. The reference aliquot was placed in a sterile tube and processed for QBC within 6 hours. A bacteriology swab was then immersed in the urine for 5 seconds and returned to the charcoal Amies media container. The urine samples in the sterile tube and bacteriology swab were stored at room temperature for 48 hours and processed for QBC. o Results Thirty-seven of the samples were positive on reference culture with a total of 42 bacterial isolates. Samples stored in sterile tube and bacteriology swab had identical sensitivity and specificity for detection of bacteriuria (94.7% and 100%, respectively) with very good agreement (κ = 0.92; 95% CI 0.81 to 1.00). Agreement between the bacterial species of the reference sample and the bacteriology swab was higher (κ= 0.85; 95% CI 0.71 to 0.99) than compared to the sterile tube (κ= 0.78; 95% CI 0.62 to 0.94), but the overlapping confidence intervals mean improved agreement cannot be inferred. o Clinical significance Bacteriology swabs stored in Amies charcoal transport media should be considered an alternative method to preserve canine urine sample when immediate processing for QBC is not possible. The sensitivity of culturing plain urine, stored for 48 hours in a sterile tube, for detection of bacteriuria, was higher than previously reported.
dc.description.sponsorshipAnimal Health Trust, Newmarket
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherWiley
dc.rightsAll rights reserved
dc.rights.uri
dc.titleThe utility of Amies charcoal bacteriology swabs for storage of canine urine prior to culture.en
dc.typeArticle
prism.publicationDate2020en
prism.publicationNameThe Journal of small animal practiceen
dc.identifier.doi10.17863/CAM.59979
dcterms.dateAccepted2020-10-26en
rioxxterms.versionofrecord10.1111/jsap.13269en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-12-04en
dc.contributor.orcidOlivares, G [0000-0002-6398-6407]
dc.contributor.orcidManzanilla, E [0000-0002-2301-2560]
dc.contributor.orcidWarland, James [0000-0003-1060-1865]
dc.identifier.eissn1748-5827
rioxxterms.typeJournal Article/Reviewen
cam.orpheus.successMon Dec 14 07:30:38 GMT 2020 - Embargo updated*
cam.orpheus.counter7*
rioxxterms.freetoread.startdate2021-12-04


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