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dc.contributor.authorHyvärinen, Tanja
dc.contributor.authorHyysalo, Anu
dc.contributor.authorKapucu, Fikret Emre
dc.contributor.authorAarnos, Laura
dc.contributor.authorVinogradov, Andrey
dc.contributor.authorEglen, Stephen J.
dc.contributor.authorYlä-Outinen, Laura
dc.contributor.authorNarkilahti, Susanna
dc.date.accessioned2020-11-19T16:19:43Z
dc.date.available2020-11-19T16:19:43Z
dc.date.issued2019-11-20
dc.date.submitted2019-08-29
dc.identifier.others41598-019-53647-8
dc.identifier.other53647
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/313118
dc.descriptionFunder: Tampereen Yliopisto (University of Tampere); doi: https://doi.org/10.13039/501100004371
dc.descriptionFunder: Tekes (Finnish Funding Agency for Innovation); doi: https://doi.org/10.13039/501100003406
dc.description.abstractAbstract: Human pluripotent stem cell (hPSC)-derived neurons provide exciting opportunities for in vitro modeling of neurological diseases and for advancing drug development and neurotoxicological studies. However, generating electrophysiologically mature neuronal networks from hPSCs has been challenging. Here, we report the differentiation of functionally active hPSC-derived cortical networks on defined laminin-521 substrate. We apply microelectrode array (MEA) measurements to assess network events and compare the activity development of hPSC-derived networks to that of widely used rat embryonic cortical cultures. In both of these networks, activity developed through a similar sequence of stages and time frames; however, the hPSC-derived networks showed unique patterns of bursting activity. The hPSC-derived networks developed synchronous activity, which involved glutamatergic and GABAergic inputs, recapitulating the classical cortical activity also observed in rodent counterparts. Principal component analysis (PCA) based on spike rates, network synchronization and burst features revealed the segregation of hPSC-derived and rat network recordings into different clusters, reflecting the species-specific and maturation state differences between the two networks. Overall, hPSC-derived neural cultures produced with a defined protocol generate cortical type network activity, which validates their applicability as a human-specific model for pharmacological studies and modeling network dysfunctions.
dc.languageen
dc.publisherNature Publishing Group UK
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectArticle
dc.subject/631/1647/1453/2205
dc.subject/631/532/2064/2158
dc.subject/631/532/1360
dc.subject/13/100
dc.subject/13/1
dc.subject/14/19
dc.subject/120
dc.subject/9/30
dc.subjectarticle
dc.titleFunctional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures
dc.typeArticle
dc.date.updated2020-11-19T16:19:42Z
prism.issueIdentifier1
prism.publicationNameScientific Reports
prism.volume9
dc.identifier.doi10.17863/CAM.60221
dcterms.dateAccepted2019-11-01
rioxxterms.versionofrecord10.1038/s41598-019-53647-8
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidNarkilahti, Susanna [0000-0002-7602-4418]
dc.identifier.eissn2045-2322
pubs.funder-project-idSuomen Kulttuurirahasto (Finnish Cultural Foundation) (00170805)
pubs.funder-project-idAcademy of Finland (Suomen Akatemia) (286990, 312414)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)