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dc.contributor.authorPalaniyandi, Kannan
dc.contributor.authorKumar, Narender
dc.contributor.authorVeerasamy, Maroudam
dc.contributor.authorKabir Refaya, Ahmed
dc.contributor.authorDolla, Chandrakumar
dc.contributor.authorBalaji, Subramanyam
dc.contributor.authorBaskaran, Dhanaraj
dc.contributor.authorThiruvengadam, Kannan
dc.contributor.authorRajendran, Ananthi
dc.contributor.authorNarayanan, Sujatha
dc.contributor.authorRaj, Dhinakar
dc.contributor.authorSwaminathan, Soumya
dc.contributor.authorPeacock, Sharon J.
dc.date.accessioned2020-11-28T16:12:08Z
dc.date.available2020-11-28T16:12:08Z
dc.date.issued2019-11-29
dc.date.submitted2019-03-29
dc.identifier.others41598-019-54268-x
dc.identifier.other54268
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/313502
dc.descriptionFunder: UK Medical Research Council, grant Reference number (MR/N501864/1)
dc.description.abstractAbstract: The major human pathogen Mycobacterium tuberculosis is rarely reported to cause disease in other animals. Cases in livestock are thought to occur through contact with infected handlers, but previous studies evaluating putative livestock-human transmission used typing techniques with limited resolution. Here, we undertook cross-sectional surveillance for tuberculosis in 271 livestock handlers and 167 cattle on three farms in Chennai, India and defined the relatedness of cultured isolates using whole genome sequencing. Humans and livestock were screened for active mycobacterial infection, and opportunistic post-mortem examination was performed on comparative intradermal test-positive cattle that died. Four cattle and 6 handlers on two farms were culture-positive for M. tuberculosis; M. bovis was not isolated. All 10 isolates (one from each case) belonged to Lineage 1. Pairwise genome comparisons of single nucleotide polymorphism (SNP) differences ranged from 1 to 600 SNPs, but 3 isolate pairs were less than 5 SNPs different. Two pairs were from handlers and the third pair were from two cattle on the same farm. The minimum pairwise SNP difference between a cattle and human isolate was >250 SNPs. Our study confirms the presence of M. tuberculosis infection in cattle in India, sequencing of which characterised relatedness between human and cattle-derived isolates.
dc.languageen
dc.publisherNature Publishing Group UK
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectArticle
dc.subject/631/326/107
dc.subject/631/326/421
dc.subject/45/23
dc.subject/45/22
dc.subjectarticle
dc.titleIsolation and comparative genomics of Mycobacterium tuberculosis isolates from cattle and their attendants in South India
dc.typeArticle
dc.date.updated2020-11-28T16:12:08Z
prism.issueIdentifier1
prism.publicationNameScientific Reports
prism.volume9
dc.identifier.doi10.17863/CAM.60606
dcterms.dateAccepted2019-10-21
rioxxterms.versionofrecord10.1038/s41598-019-54268-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidKabir Refaya, Ahmed [0000-0001-7580-5477]
dc.contributor.orcidPeacock, Sharon J. [0000-0002-1718-2782]
dc.identifier.eissn2045-2322


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)