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dc.contributor.authorKlingstedt, Therése
dc.contributor.authorGhetti, Bernardino
dc.contributor.authorHolton, Janice L.
dc.contributor.authorLing, Helen
dc.contributor.authorNilsson, K. Peter R.
dc.contributor.authorGoedert, Michel
dc.date.accessioned2020-12-02T16:12:00Z
dc.date.available2020-12-02T16:12:00Z
dc.date.issued2019-12-03
dc.date.submitted2019-10-29
dc.identifier.others40478-019-0840-1
dc.identifier.other840
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/313910
dc.description.abstractAbstract: Synucleinopathies [Parkinson’s disease with or without dementia, dementia with Lewy bodies and multiple system atrophy] are neurodegenerative diseases that are defined by the presence of filamentous α-synuclein inclusions. We investigated the ability of luminescent conjugated oligothiophenes to stain the inclusions of Parkinson’s disease and multiple system atrophy. They stained the Lewy pathology of Parkinson’s disease and the glial cytoplasmic inclusions of multiple system atrophy. Spectral analysis of HS-68-stained inclusions showed a red shift in multiple system atrophy, but the difference with Parkinson’s disease was not significant. However, when inclusions were double-labelled for HS-68 and an antibody specific for α-synuclein phosphorylated at S129, they could be distinguished based on colour shifts with blue designated for Parkinson’s disease and red for multiple system atrophy. The inclusions of Parkinson’s disease and multiple system atrophy could also be distinguished using fluorescence lifetime imaging. These findings are consistent with the presence of distinct conformers of assembled α-synuclein in Parkinson’s disease and multiple system atrophy.
dc.languageen
dc.publisherBioMed Central
dc.subjectResearch
dc.subjectα-Synuclein
dc.subjectLuminescent conjugated oligothiophene
dc.subjectMultiple system atrophy
dc.subjectNeurodegeneration
dc.subjectParkinson’s disease
dc.subjectSpectral analysis
dc.titleLuminescent conjugated oligothiophenes distinguish between α-synuclein assemblies of Parkinson’s disease and multiple system atrophy
dc.typeArticle
dc.date.updated2020-12-02T16:12:00Z
prism.issueIdentifier1
prism.publicationNameActa Neuropathologica Communications
prism.volume7
dc.identifier.doi10.17863/CAM.61013
dcterms.dateAccepted2019-10-29
rioxxterms.versionofrecord10.1186/s40478-019-0840-1
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn2051-5960
pubs.funder-project-idFondation pour la Recherche sur Alzheimer (N/A)
pubs.funder-project-idUK Medical Research Council (MC_U105184291)
pubs.funder-project-idVetenskapsrådet (2016-00748)
pubs.funder-project-idNational Institute on Aging (PHS P30 AG010133)
pubs.funder-project-idMultiple System Atrophy Trust (N/A, N/A)
pubs.funder-project-idMultiple System Atrophy Coalition (N/A)
pubs.funder-project-idFund Sophia managed by the King Baudouin Foundation (N/A, N/A)
pubs.funder-project-idCBD Solutions (512385)


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