High-resolution label-free 3D mapping of extracellular pH of single living cells
Authors
Zhang, Yanjun
Hong, Sung Pil
Liu, Fengjie
Bednarska, Joanna
Novak, Pavel
Shevchuk, Andrew
Gopal, Sahana
Barozzi, Iros
Sakai, Hideki
Suguru, Yoshimoto
Fujii, Takuto
Erofeev, Alexander
Gorelkin, Peter
Majouga, Alexander
Weiss, Dominik J.
Edwards, Christopher
Klenerman, David
Korchev, Yuri
Publication Date
2019-12-06Journal Title
Nature Communications
Publisher
Nature Publishing Group UK
Volume
10
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Zhang, Y., Takahashi, Y., Hong, S. P., Liu, F., Bednarska, J., Goff, P. S., Novak, P., et al. (2019). High-resolution label-free 3D mapping of extracellular pH of single living cells. Nature Communications, 10 (1) https://doi.org/10.1038/s41467-019-13535-1
Abstract
Abstract: Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.
Keywords
Article, /631/57/2282, /631/57/2265, /639/638, /639/925, /147, /123, /13, /14, /14/10, article
Identifiers
s41467-019-13535-1, 13535
External DOI: https://doi.org/10.1038/s41467-019-13535-1
This record's URL: https://www.repository.cam.ac.uk/handle/1810/314803
Rights
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/
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