RORγt + Treg to Th17 ratios correlate with susceptibility to Giardia infection
Kühl, Anja A.
Heimesaat, Markus M.
Nature Publishing Group UK
MetadataShow full item record
Yordanova, I. A., Cortés, A., Klotz, C., Kühl, A. A., Heimesaat, M. M., Cantacessi, C., Hartmann, S., & et al. (2019). RORγt + Treg to Th17 ratios correlate with susceptibility to Giardia infection. Scientific Reports, 9 (1) https://doi.org/10.1038/s41598-019-56416-9
Funder: Fundacion Alfonso Martin Escudero
Funder: RCUK | Biotechnology and Biological Sciences Research Council (BBSRC); doi: https://doi.org/10.13039/501100000268
Funder: Isaac Newton Trust; doi: https://doi.org/10.13039/501100004815
Abstract: Infections with Giardia are among the most common causes of food and water-borne diarrheal disease worldwide. Here, we investigated Th17, Treg and IgA responses, and alterations in gut microbiota in two mouse lines with varying susceptibility to Giardia muris infection. Infected BALB/c mice shed significantly more cysts compared with C57BL/6 mice. Impaired control of infection in BALB/c mice was associated with lower Th17 activity and lower IgA levels compared with C57BL/6 mice. The limited metabolic activity, proliferation and cytokine production of Th17 cells in BALB/c mice was associated with higher proportions of intestinal Foxp3+RORγt+ regulatory T cells and BALB/c mice developed increased RORγt+ Treg:Th17 ratios in response to G. muris infection. Furthermore, G. muris colonization led to a significantly reduced evenness in the gut microbial communities of BALB/c mice. Our data indicate that differential susceptibility to Giardia infections may be related to RORγt+ Treg controlling Th17 activity and that changes in the microbiota composition upon Giardia infection partially depend on the host background.
Article, /631/250/2152/1566, /631/250/347, /631/326/417/2546, /9, /13, /13/1, /13/21, /13/31, /14/34, /14/63, /38/77, /38/90, /38/91, /64/60, article
Deutsche Forschungsgemeinschaft (German Research Foundation) (GRK2046, GRK2046, GRK2046)
Bundesministerium für Bildung und Forschung (Federal Ministry of Education and Research) (IP7/01KI1725D)
External DOI: https://doi.org/10.1038/s41598-019-56416-9
This record's URL: https://www.repository.cam.ac.uk/handle/1810/315637
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/