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dc.contributor.authorShafiq, Arooj
dc.contributor.authorCampbell, Louise J.
dc.contributor.authorOwen, Darerca
dc.contributor.authorMott, Helen R.
dc.date.accessioned2021-01-07T16:23:36Z
dc.date.available2021-01-07T16:23:36Z
dc.date.issued2020-01-08
dc.date.submitted2019-12-04
dc.identifier.issn1874-2718
dc.identifier.others12104-019-09925-7
dc.identifier.other9925
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/315838
dc.description.abstractAbstract: The Ral proteins (RalA and RalB) are small G proteins of the Ras family that have been implicated in exocytosis, endocytosis, transcriptional regulation and mitochondrial fission, as well as having a role in tumourigenesis. RalA and RalB are activated downstream of the master regulator, Ras, which causes the nucleotide exchange of GDP for GTP. Here we report the 1H, 15 N and 13C resonance assignments of RalA in its active form bound to the GTP analogue GMPPNP. We also report the backbone assignments of RalA in its inactive, GDP-bound form. The assignments give insight into the switch regions, which change conformation upon nucleotide exchange. These switch regions are invisible in the spectra of the active, GMPPNP bound form but the residues proximal to the switches can be monitored. RalA is also an important drug target due to its over activation in some cancers and these assignments will be extremely useful for NMR-based screening approaches.
dc.languageen
dc.publisherSpringer Netherlands
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectArticle
dc.subjectRas
dc.subjectGtpase
dc.subjectRal
dc.subjectSignalling
dc.subjectG Protein
dc.titleNMR resonance assignments for the active and inactive conformations of the small G protein RalA
dc.typeArticle
dc.date.updated2021-01-07T16:23:36Z
prism.endingPage91
prism.issueIdentifier1
prism.publicationNameBiomolecular NMR Assignments
prism.startingPage87
prism.volume14
dc.identifier.doi10.17863/CAM.62949
dcterms.dateAccepted2019-12-26
rioxxterms.versionofrecord10.1007/s12104-019-09925-7
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidOwen, Darerca [0000-0003-0978-5425]
dc.contributor.orcidMott, Helen R. [0000-0002-7890-7097]
dc.identifier.eissn1874-270X
pubs.funder-project-idMedical Research Council (MR/J007803/1)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)