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Stages, scaffolds and strings in the spatial organisation of non-homologous end joining: Insights from X-ray diffraction and Cryo-EM.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Chaplin, Amanda K 
Stavridi, Antonia Kefala 
Appleby, Robert 
Hnizda, Ales 

Abstract

Non-homologous end joining (NHEJ) is the preferred pathway for the repair of DNA double-strand breaks in humans. Here we describe three structural aspects of the repair pathway: stages, scaffolds and strings. We discuss the orchestration of DNA repair to guarantee robust and efficient NHEJ. We focus on structural studies over the past two decades, not only using X-ray diffraction, but also increasingly exploiting cryo-EM to investigate the macromolecular assemblies.

Description

Keywords

Cryo-EM, DNA repair, Double-strand breaks, NHEJ, Scaffolds, Stages, Strings, X-ray diffraction, Cryoelectron Microscopy, DNA Breaks, Double-Stranded, DNA End-Joining Repair, DNA Repair, Humans, X-Ray Diffraction

Journal Title

Prog Biophys Mol Biol

Conference Name

Journal ISSN

0079-6107
1873-1732

Volume Title

163

Publisher

Elsevier BV

Rights

All rights reserved
Sponsorship
Wellcome Trust (093167/Z/10/Z)
Wellcome Trust (200814/Z/16/Z)