Stages, scaffolds and strings in the spatial organisation of non-homologous end joining: Insights from X-ray diffraction and Cryo-EM.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Liang, Shikang https://orcid.org/0000-0002-6930-7882
Chaplin, Amanda K
Stavridi, Antonia Kefala
Appleby, Robert
Hnizda, Ales
Abstract
Non-homologous end joining (NHEJ) is the preferred pathway for the repair of DNA double-strand breaks in humans. Here we describe three structural aspects of the repair pathway: stages, scaffolds and strings. We discuss the orchestration of DNA repair to guarantee robust and efficient NHEJ. We focus on structural studies over the past two decades, not only using X-ray diffraction, but also increasingly exploiting cryo-EM to investigate the macromolecular assemblies.
Description
Keywords
Cryo-EM, DNA repair, Double-strand breaks, NHEJ, Scaffolds, Stages, Strings, X-ray diffraction, Cryoelectron Microscopy, DNA Breaks, Double-Stranded, DNA End-Joining Repair, DNA Repair, Humans, X-Ray Diffraction
Journal Title
Prog Biophys Mol Biol
Conference Name
Journal ISSN
0079-6107
1873-1732
1873-1732
Volume Title
163
Publisher
Elsevier BV
Publisher DOI
Rights
All rights reserved
Sponsorship
Wellcome Trust (093167/Z/10/Z)
Wellcome Trust (200814/Z/16/Z)
Wellcome Trust (200814/Z/16/Z)