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dc.contributor.authorZeka, Keti
dc.contributor.authorMarrazzo, Pasquale
dc.contributor.authorMicucci, Matteo
dc.contributor.authorRuparelia, Ketan C
dc.contributor.authorArroo, Randolph RJ
dc.contributor.authorMacchiarelli, Guido
dc.contributor.authorAnnarita Nottola, Stefania
dc.contributor.authorContinenza, Maria Adelaide
dc.contributor.authorChiarini, Alberto
dc.contributor.authorAngeloni, Cristina
dc.contributor.authorHrelia, Silvana
dc.contributor.authorBudriesi, Roberta
dc.date.accessioned2021-02-02T00:30:17Z
dc.date.available2021-02-02T00:30:17Z
dc.date.issued2020-11-09
dc.identifier.issn2076-3921
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/316939
dc.description.abstractThe petals of the saffron crocus (Crocus sativus L.) are considered a waste material in saffron production, but may be a sustainable source of natural biologically active substances of nutraceutical interest. The aim of this work was to study the cardiovascular effects of kaempferol and crocin extracted from saffron petals. The antiarrhythmic, inotropic, and chronotropic effects of saffron petal extract (SPE), kaempferol, and crocin were evaluated through in vitro biological assays. The antioxidant activity of kaempferol and crocin was investigated through the 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) assay using rat cardiomyoblast cell line H9c2. The MTT assay was applied to assess the effects of kaempferol and crocin on cell viability. SPE showed weak negative inotropic and chronotropic intrinsic activities but a significant intrinsic activity on smooth muscle with a potency on the ileum greater than on the aorta: EC50 = 0.66 mg/mL versus EC50 = 1.45 mg/mL. Kaempferol and crocin showed a selective negative inotropic activity. In addition, kaempferol decreased the contraction induced by KCl (80 mM) in guinea pig aortic and ileal strips, while crocin had no effect. Furthermore, following oxidative stress, both crocin and kaempferol decreased intracellular ROS formation and increased cell viability in a concentration-dependent manner. The results indicate that SPE, a by-product of saffron cultivation, may represent a good source of phytochemicals with a potential application in the prevention of cardiovascular diseases.
dc.format.mediumElectronic
dc.languageeng
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleActivity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System.
dc.typeArticle
prism.issueIdentifier11
prism.publicationDate2020
prism.publicationNameAntioxidants (Basel)
prism.volume9
dc.identifier.doi10.17863/CAM.64051
dcterms.dateAccepted2020-11-06
rioxxterms.versionofrecord10.3390/antiox9111102
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-11-09
dc.contributor.orcidZeka, Keti [0000-0002-3727-9522]
dc.contributor.orcidMarrazzo, Pasquale [0000-0001-6644-5828]
dc.contributor.orcidMicucci, Matteo [0000-0003-3525-1531]
dc.contributor.orcidArroo, Randolph RJ [0000-0002-0672-7927]
dc.contributor.orcidMacchiarelli, Guido [0000-0002-9182-0586]
dc.contributor.orcidAngeloni, Cristina [0000-0002-6731-6187]
dc.contributor.orcidHrelia, Silvana [0000-0001-7857-4512]
dc.contributor.orcidBudriesi, Roberta [0000-0002-8454-9740]
dc.identifier.eissn2076-3921
rioxxterms.typeJournal Article/Review
cam.issuedOnline2020-11-09


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International